PXD063776 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state |
| Description | T helper 17 (Th17) cells are heterogeneous; homeostatic Th17 (Th17Hom) cells maintain tissue homeostasis and pro-inflammatory Th17 (Th17Inf) cells drive autoimmune inflammation. IL-23 drives Th17Inf, but the signals that maintain Th17Hom remain unclear. Here, we found that differential glucocorticoid (GC) production downstream of CYP11A1, a critical enzyme for steroidogenesis, distinguished Th17Hom cells from Th17Inf cells. Although TCR signaling opened the Cyp11a1 locus, TGFβ1 and IL-6, key cytokines for Th17Hom cell differentiation, maintained and amplified Cyp11a1. Th17Hom cell-derived GC signaled through the glucocorticoid receptor (GR), which was higher in Th17Hom cells compared to Th17Inf cells, thereby forming a circuit that maintained the homeostatic state. Integration of multi-omics data from CYP11A1- and GR-deficient Th17Hom cells revealed a gene network underlying this circuit. TGFβ1, a key node in the network, restored GC sensing to Th17Inf. We thus identify a GC signaling circuit that maintains Th17 homeostasis with implications for treating Th17-mediated autoimmunity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-12 |
| AnnouncementXML | Submission_2026-06-12_11:55:51.116.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuyang Han |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-08 12:24:56 | ID requested | |
| ⏵ 1 | 2026-06-12 11:55:51 | announced | |
Publication List
Keyword List
| submitter keyword: Th17, glucocorticoid,CYP11A1, homeostasis, TGFβ1, CNS autoimmunity |
Contact List
| Ana Carrizosa Anderson |
|---|
| contact affiliation | The Gene Lay Institute of Immunology and Inflammation Harvard Medical School and Brigham and Women's Hospital |
| contact email | acanderson@bwh.harvard.edu |
| lab head | |
| Yuyang Han |
|---|
| contact affiliation | Brigham and Women's Hospital |
| contact email | yhan8@bwh.harvard.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063776 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063776
- Label: PRIDE project
- Name: A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state
