PXD063771-1

PXD063771 is an original dataset announced via ProteomeXchange.

Title	Dynamic Release of Exosomes After Opening of the Blood-Brain Barrier Predicts Glioblastoma Susceptibility to Paclitaxel
Description	Liquid biopsies hold promise to improve the diagnosis, response to therapy, and ultimately guide the management of cancer patients, however implementation of this approach in brain tumors has proven challenging due to the limited passage of molecules across the blood-brain barrier (BBB). We recently reported results from a phase I clinical trial in which the BBB was transiently opened in glioblastoma (GBM) patients using skull-implantable low-intensity ultrasound combined with microbubbles (LIPU/MB), performed every 3 weeks with paclitaxel administration until disease progression (NCT04528680). Here, we investigate circulating exosomes, a potential biomarker for susceptibility to treatment, as an exploratory endpoint of this trial. After developing and validating a microfluidic device designed to capture tumor-specific exosomes in glioblastoma patients (GlioExoChip), we studied longitudinal changes in exosome concentrations during LIPU/MB-based BBB disruption and chemotherapy delivery. Exosomes were characterized using nanoparticle tracking analysis, proteomics, western blot, and scanning electron microscopy. GlioExoChip had high capture efficiency for GBM-specific exosomes with phosphatidylserine and Annexin-V chemistry. Longitudinal analysis of exosome changes after BBB opening correlated with MRI enhancement among patients. In vitro, paclitaxel-susceptible glioma cells treated with this drug exhibited apoptosis and exosome release. In concordance, we found changes in exosome release following initiation of paclitaxel with LIPU/MB correlated with overall survival in GBM patients. Our work presents an efficient microfluidic platform for capturing of circulating GBM exosomes , prospectively characterizes the release of tumor material after the opening of the BBB in a clinical trial , and demonstrates that longitudinal liquid biopsies can provide practical insights to improve the care of brain tumor patients
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-21_16:09:39.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abha Kumari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 10:01:13	ID requested
⏵ 1	2025-12-21 16:09:40	announced

10.1038/s41467-025-65681-4;

Youngblood MW, Kumari A, Kang YT, Gould A, Habashy K, Gomez M, Lingamarla H, Morey T, Chen L, Congivaram H, Ward R, Zhang H, Sears TK, McCortney K, Pituch KC, Torres Ponce EM, Zarrieneh A, Nieves M, Vandermolen S, Primdahl D, Dixit K, Lukas RV, Kumthekar P, Dmello C, Bouchoux G, Canney M, Amidei C, Stupp R, Nagrath S, Sonabend AM, Dynamic release of extracellular particles after opening of the blood-brain barrier predicts glioblastoma susceptibility to paclitaxel. Nat Commun, 16(1):11045(2025) [pubmed]

submitter keyword: exosomes, microfluidics, blood brain barrier,Glioblastoma

Abha Kumari
contact affiliation	Sunitha Nagrath Lab, 2800 Plymouth Rd, NCRC Building 10, Ann Arbor, MI- 48109
contact email	abhak@umich.edu
lab head
Abha Kumari
contact affiliation	University of Michigan Ann Arbor
contact email	abhak@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063771
     1. Label: PRIDE project
     2. Name: Dynamic Release of Exosomes After Opening of the Blood-Brain Barrier Predicts Glioblastoma Susceptibility to Paclitaxel