PXD063745 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Intralysosomal pathogens differentially influence the proteolytic potential of their niche |
| Description | Avoiding lysosomal degradation is vital to the success of intracellular pathogens. The Gram-negative bacterium C. burnetii and protozoan parasites of the Leishmania genus share the unique ability to replicate within a host phagolysosomal compartment, though the exact mechanisms utilised to withstand this hostile environment are not clearly defined. We recently reported that C. burnetii removes the lysosomal protease cathepsin B during infection of mammalian cells. Here, we aimed to determine if this virulence strategy was also employed by the intralysosomal pathogen L. mexicana. The activity of specific cathepsins was analysed, using immunoblotting and protease activity-based probes, and the changes observed during infection with C. burnetii were not observed during L. mexicana infection. Co-infection of THP-1 macrophage like cells with both pathogens resulted in a proteolytic and secretory phenotype consistent with C. burnetii infection, suggesting that C. burnetii-induced remodelling of the lysosome is not influenced by L. mexicana. The host cell proteome and secretome of L. mexicana infected cells was defined using mass spectrometry. This confirmed that, unlike C. burnetii, L. mexicana does not induce increased abundance of lysosomal proteins either intracellularly or in the extracellular milieu. Collectively, this study reveals that although C. burnetii and L. mexicana reside in a phagolysosomal intracellular niche, they employ divergent mechanisms to survive within this hostile compartment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-22_06:20:38.668.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lauren Bird |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-07 16:27:13 | ID requested | |
| ⏵ 1 | 2025-12-22 06:20:39 | announced | |
Publication List
Keyword List
| submitter keyword: Leishmania mexicana, coinfection,Coxiella burnetii, cathepsin B, cathepsin D |
Contact List
| Prof Hayley Newton |
|---|
| contact affiliation | Infection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, VIC 3800, Australia |
| contact email | hayley.newton@monash.edu |
| lab head | |
| Lauren Bird |
|---|
| contact affiliation | The University of Melbourne/Monash University |
| contact email | lauren.bird@monash.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063745
- Label: PRIDE project
- Name: Intralysosomal pathogens differentially influence the proteolytic potential of their niche
