PXD063724-1

PXD063724 is an original dataset announced via ProteomeXchange.

Title	MAP1B as nuclear neural stem cell fate determinant involved in disease
Description	Cellular differentiation and morphogenesis rely on both cytoskeletal remodeling and transcriptional programs to drive cell fate decisions. Their coordination is critical for proper lineage progression, yet if and how cytoskeletal elements can regulate fate in the nucleus is largely unknown. To explore this comprehensively, we profiled the nuclear proteome of human and murine neural stem cells (NSCs). Cytoskeletal proteins were enriched among the most abundant nuclear components, with a predominance of microtubule (MT)-interacting proteins, including the MT-associated protein MAP1B. MAP1B mutations have been identified in patients with periventricular heterotopia (PH), linked to neuron migration deficits. We found that MAP1B translocates to the nucleus in NSCs, where it interacts with the BAF chromatin remodeling complex to regulate NSC identity and differentiation. Perturbing MAP1B cytoplasmic-to-nuclear balance in NSCs leads to the generation of ectopic neurons in the mouse cerebral cortex, as found in PH patients harboring MAP1B mutations. Patient iPSC-derived brain organoids show nuclear enrichment of mutant MAP1B resulting in enhanced BAF chromatin binding along with neuronal heterotopia. Thus, MAP1B acts as a novel nuclear regulator of NSC fate, highlighting the key role of cytoskeletal elements in the nucleus for fate specification, and how disruption of this process can lead to disease.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_03:35:29.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 07:43:51	ID requested
⏵ 1	2026-06-08 03:35:29	announced

10.1016/J.CELL.2026.05.019;

submitter keyword: LC-MS/MS, NSCs, MAP1B

Magdalena Götz
contact affiliation	Division of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-Universität, Planegg-Martinsried, Germany. Institute of Stem Cell Research, Helmholtz Center Munich, Planegg-Martinsried, Germany.
contact email	magdalena.goetz@helmholtz-munich.de
lab head
Juliane Merl-Pham
contact affiliation	Metabolomics and Proteomics Core, Helmholtz Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063724

PRIDE project URI

• PRIDE
  1. PXD063724
     1. Label: PRIDE project
     2. Name: MAP1B as nuclear neural stem cell fate determinant involved in disease