PXD063714-1

PXD063714 is an original dataset announced via ProteomeXchange.

Title	ABCF Protein-Mediated Resistance Shapes Bacterial Responses Based on Antibiotic Type and Concentration
Description	Antibiotic resistance (ARE) ABCF proteins are widespread, genome-encoded resistance factors in Gram-positive bacteria that rescue ribosomes stalled by antibiotics. In this study, we integrate genetic, proteomic, metabolomic, and biochemical approaches to reveal that ARE5 subfamily proteins, TiaA and Are5sc, leverage their resistance functions to regulate responses to lincosamides, streptogramins A, and pleuromutilins (LSaP) in Streptomyces coelicolor. While both Are5sc and TiaA are critical for the activation of actinorhodin production in response to LSaP antibiotics, they play distinct roles at the proteome level, determined by their differing resistance profiles and temporally regulated expression. This expression is transcriptionally induced by LSaP antibiotics via an attenuation mechanism and is reciprocally fine-tuned by the resistance functions of the ARE5 paralogs reflecting the specific type and concentration of the antibiotic. Are5sc modulates early adaptive responses by fine-tuning the WblC regulon across a broad range of antibiotic concentrations. In contrast, TiaA is induced later, at higher antibiotic concentrations, where it suppresses global antibiotic stress responses, specifically targeting pleuromutilins produced by higher fungi, reflecting a distinct ecological context compared to lincosamides and streptogramins, which are primarily produced by actinomycetes. Our study provides the first example of ARE ABCF proteins shaping antibiotic response at the translational level, expanding our understanding of the regulatory functions of these ubiquitous proteins.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-28_00:31:22.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Jasprova
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-07 01:44:33	ID requested
⏵ 1	2025-07-28 00:31:22	announced

Dataset with its publication pending

submitter keyword: Streptomyces coelicolor,ARE ACBF proteins, antibiotic resistance

GABRIELA BALÍKOVÁ NOVOTNÁ
contact affiliation	Laboratory of Antibiotic Resistance and Microbial Metabolomics, Kamenik & Balikova Novotna Lab, Institute of Microbiology of the Czech Academy of Sciences, Czech Republic
contact email	gnovotna@biomed.cas.cz
lab head
Jana Jasprova
contact affiliation	Charles University, Faculty of Science
contact email	vanikova@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063714

PRIDE project URI

• PRIDE
  1. PXD063714
     1. Label: PRIDE project
     2. Name: ABCF Protein-Mediated Resistance Shapes Bacterial Responses Based on Antibiotic Type and Concentration