PXD063658 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling |
| Description | An inability to replicate the genome can cause replication stress and genome instability. Here, we develop BLOCK-ID, a proteomic method to identify and visualize proteins at stressed replication forks. This approach successfully identified novel mediators of the replication stress response, including the chromatin acetylation reader protein TRIM24. We uncovered a crucial role for TRIM24 in coordinating Alternative Lengthening of Telomeres (ALT), a replication stress-directed telomere extension mechanism. Our data reveal that TRIM24 is recruited to telomeres via a p300/CBP-dependent acetylation chromatin signaling cascade to organize the assembly of ALT-associated PML bodies (APBs) and promote de novo telomere DNA synthesis. Unexpectedly, tethering of TRIM24 at telomeres was sufficient to stimulate de novo telomere DNA synthesis in a SUMO-dependent, but p300/CBP- and PML-independent manner. Collectively, these findings uncover an indispensable epigenetic signaling pathway involving TRIM24 and p300/CBP that mediate ALT telomere maintenance. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-31 |
| AnnouncementXML | Submission_2026-08-30_16:11:47.753.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | chanhyun na |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-06 07:25:13 | ID requested | |
| ⏵ 1 | 2026-08-30 16:11:48 | announced | |
Publication List
| Kim D, Bhargava R, Wang SC, Tseng WC, Lee D, Patel R, Oh S, Bowman RW, Smith BA, Kim M, Na CH, O'Sullivan RJ, Miller KM, TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling. Mol Cell, 85(14):2636-2653.e8(2025) [pubmed] |
| 10.1016/j.molcel.2025.06.009; |
Keyword List
| submitter keyword: TRIM24 |
| BLOCK-ID |
| Replication stress |
| ALT |
| Telomeres |
| SUMOylation |
Contact List
| Kyle M. Miller |
|---|
| contact affiliation | Department of Radiation Oncology, School of Medicine and the Winship Cancer Institute, Emory University |
| contact email | kyle.m.miller@emory.edu |
| lab head | |
| chanhyun na |
|---|
| contact affiliation | Johns Hopkins University |
| contact email | chanhyun.na@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD063658 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063658
- Label: PRIDE project
- Name: TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling
