⮝ Full datasets listing
PXD063648-1
PXD063648 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An Adipose mTORC1 Effector Links Ribosomal Protein Translation to Lifespan |
| Description | Mechanistic target of rapamycin complex 1 (mTORC1) senses nutrient availability to orchestrate metabolic processes critical for physiological homeostasis and organismal aging. While mTORC1 preferentially regulates the translation of 5′-terminal oligopyrimidine (TOP) motif-containing mRNAs that predominantly encode ribosomal proteins (RPs) via the translational repressor 4E-BP, this mTORC1 function is resistant to rapamycin inhibition. TOP mRNAs are exceptionally abundant, thus imposing a major translational burden on cells; yet how their translation is physiologically tuned and linked to longevity remain unexplored. Here we identify Lsp2, originally known as a storage protein, as an adipose effector and feedback activator of mTORC1 that modulates lifespan in Drosophila. Lsp2 expression is induced by essential amino acids (EAAs) via mTORC1 and gated by additional signals of nutrient sufficiency. Genetic ablation of Lsp2 robustly extends lifespan without impairing key life-history traits such as reproduction. Translatomic profiling shows that loss of Lsp2 selectively reduces global TOP mRNA translation in a 4E-BP–dependent manner, thereby extending lifespan via a mechanism distinct from rapamycin. Evolutionarily, TOP motifs co-emerged with 4E-BP and are present in nearly all Drosophila RP mRNAs. Moreover, we demonstrate that their role in translational control extends to Drosophila. Collectively, our findings reveal a nutrient-induced physiological factor that amplifies mTORC1 output in TOP mRNA translation and regulates organismal longevity. |
| HostingRepository | iProX |
| AnnounceDate | 2025-04-30 |
| AnnouncementXML | Submission_2026-07-22_19:54:17.901.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jun Wang |
| SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-05-06 06:47:13 | ID requested | |
| ⏵ 1 | 2026-07-22 19:54:18 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Drosophila, Lsp2, Ribosomal protein |
Contact List
| Zongzhao Zhai | |
|---|---|
| contact affiliation | Hunan Normal University |
| contact email | zongzhao.zhai@foxmail.com |
| lab head | |
| Jun Wang | |
| contact affiliation | Hunan Normal University |
| contact email | 282936504@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
