PXD063566 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Subcellular Mass Spectrometry Reveals Proteome Remodeling in an Asymmetrically Dividing (Frog) Embryonic Stem Cell |
| Description | This project enhanced discovery subcellular proteomics sensitivity by integrating diaPASEF with capillary electrophoresis mass spectrometry (CE-MS). The method was developed, tested, and validated on ~200 pg of the HeLa proteome digest, ~80% of the total proteome of a single cell. Reproducible identification of at least 1,000 proteins marked robust performance to explore subcellular proteome heterogeneity as the dorsal-animal (D1) cell divides asymmetrically in the Xenopus laevis embryo to establish the D11 and D12 descendants in the 16-cell embryo. The label-free quantification (LFQ) values were normalized to compare equal proteome amounts. Relative quantification of the proteomes revealed molecular differences between the future D11 and future D12 poles of the D1 cell and the offspring cells. Functional experiments ventralizing the fertilized embryo using ultraviolet (UV) light revealed corroborated the proteome profiles and helped decipher the origin of the gradients in the context of dorsal-ventral patterning. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-10 |
| AnnouncementXML | Submission_2026-01-09_17:23:01.876.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Peter Nemes |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: NEWT:8355; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-02 11:28:09 | ID requested | |
| ⏵ 1 | 2026-01-09 17:23:02 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics, mass spectrometry, differentiation, profiling,Single cell, asymmetric division, capillary electrophoresis, diaPASEF, quantification |
Contact List
| Peter Nemes |
|---|
| contact affiliation | University of Maryland, College Park |
| contact email | nemes@umd.edu |
| lab head | |
| Peter Nemes |
|---|
| contact affiliation | University of Maryland, College Park |
| contact email | nemes@umd.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063566 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063566
- Label: PRIDE project
- Name: Subcellular Mass Spectrometry Reveals Proteome Remodeling in an Asymmetrically Dividing (Frog) Embryonic Stem Cell
