PXD063546-1

PXD063546 is an original dataset announced via ProteomeXchange.

Title	Acellular ECM Proteomics: Versican expression from lung fibroblasts is an autologous fibrotic brake
Description	The activation and accumulation of lung fibroblasts, leading to excessive extracellular matrix (ECM) deposition, is a pathogenic hallmark of Idiopathic Pulmonary Fibrosis (IPF), a lethal and currently incurable disease. The collagen-rich fibrotic ECM perpetuates fibroblast activation and accumulation, which includes the promotion of podosome formation and ECM invasion. Proteoglycans (PGs), a significant component of the interstitial ECM, fine-tune the overall tissue architecture determined by fibrous proteins and regulate several ECM-controlled signalling pathways. In this report, increased expression of Versican (VCAN ), a multifunctional PG, was detected in human and mouse pulmonary fibrosis, predominantly in monocytic cells and fibroblasts. Genetic reduction of Vcan expression in mice promoted collagen expression and modulated pulmonary ECM composition and structure, exacerbating pulmonary fibrosis and delaying its resolution. Reduced ECM Vcan levels resulted in longer, thicker, and more tangled collagen fibrils, which stimulated podosome formation in fibroblasts and their ECM invasion. Moreover, the decrease of Vcan in the ECM and the ensuing reorganisation stimulated Tenascin-C (TNC) expression from fibroblasts, which was further shown to be a potent, TLR4-dependent, autologous podosome inducer, promoting ECM invasion. Thus, Vcan expression from fibroblasts serves as an autologous fibrotic brake, controlling the underlying ECM composition, structure and mechanotransduction, and suppressing fibroblast invasion and pulmonary fibrosis.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:31:48.320.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-02 00:05:10	ID requested
⏵ 1	2026-02-22 17:31:49	announced

Kanellopoulou P, Barbayianni I, Fanidis D, Samiotaki M, Katsiouli E, Nastos D, Smyrniotis S, Shira M, Galaris A, Rinotas V, Grammenoudi S, Magkrioti C, Tomos I, Martinez Blanco A, Tremi I, Vamvakaris I, Gavara N, Havaki S, Gorgoulis V, Watanabe H, Aidinis V, Versican expression from lung fibroblasts suppresses pulmonary fibrosis. Nat Commun, 17(1):1676(2026) [pubmed]

10.1038/s41467-026-68377-5;

submitter keyword: aECM, pulmonary fibrosis

extracellular matrix (ECM)

lung fibroblasts

podosomes

ECM invasion

Versican (VCAN)

Hyaluronan (HA)

Collagens

Vasilis Aidinis
contact affiliation	Institute for Fundamental Biomedical Research, Biomedical Sciences Research Center Alexander Fleming, Athens/Greece
contact email	V.Aidinis@Fleming.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD063546

PRIDE project URI

• PRIDE
  1. PXD063546
     1. Label: PRIDE project
     2. Name: Acellular ECM Proteomics: Versican expression from lung fibroblasts is an autologous fibrotic brake