PXD063531-1

PXD063531 is an original dataset announced via ProteomeXchange.

Title	Discovery, characterization and quantification of bioactive peptides contained in Palbio porcine intestinal mucosa hy-drolysate products.
Description	Porcine intestinal mucosa hydrolysates (PIMH) are a by-product of heparin production obtained through a specific enzymatic hydrolysis process which can theoretically generate bioactive pep-tides (BAPs). This study aimed to identify, characterize and quantify BAPs in two Palbio products manufactured by Bioiberica S.A.U. (Palafolls, Spain) which are PIMH protein sources used for animal feed: Palbio® HP (PHP) and Palbio® 62 SP® (P62). Using mass spectrometry (MS)-based peptidomics, we analyzed three samples from each product, fractionated based on molecular weight (<3 kDa, 3 to 10 kDa, and >10 kDa). The <3 kDa fraction was analyzed directly, while the other two fractions were enzymatically digested before MS analysis. The workflow identified 961 peptides in PHP and 1134 in P62. Subsequent bioinformatic analysis using public databases (APD2, StraPep, AHTPDB, and BIOPEP-UWM) led to the identification of six significant BAPs in both PHP and P62, with respective quantified amounts (pg peptide/μg sample): DAVEDLESVGK (0.1626, 0.1939), EGIPPDQQRLIFAGK (0.2637, 0.1852), TITLEVEPSDTIENVK (0.3594, 0.4327), TNVPRASVPDGFLS (1.4596, 0.1898), TNVPRASVPDGFLSEL (8.0500, 0.9224), and VHVVPDQLMAF (0.0310, 0.0054). The first three BAPs are related to antimicrobial activity, while the latter three are associated with cytokine/growth factor-like, antioxidant, and immunomodulatory activities. These bioactivities align with previously reported in vivo benefits observed in animal nutrition using Palbio products. Our findings demonstrate that PHP and P62 are valuable sources of BAPs, supporting their potential role in improving animal health and performance.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:06:40.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joan Josep Bech-Serra
SpeciesList	scientific name: Sus scrofa (Pig); NCBI TaxID: 9823;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-01 05:47:35	ID requested
⏵ 1	2025-08-03 16:06:41	announced

Segarra S, de la Torre C, Bech-Serra JJ, Cucurull B, Marazuela-Duque A, Vaquero A, Mart, í, nez-Puig D, Velasco-Alvarez J, The Discovery, Characterization, and Quantification of Bioactive Peptides Contained in Palbio Porcine Intestinal Mucosa Hydrolysate Products. Int J Mol Sci, 26(14):(2025) [pubmed]

10.3390/ijms26146656;

submitter keyword: bioactive peptides

antimicrobial peptides

sustainable processing

porcine intestinal mucosa hydrolysate

porcine digestible peptides

bio-compound valorization

animal by-products

Sergi Segarra
contact affiliation	Bioiberica S.A.U., Av. Països Catalans 34, 08950 Esplugues de Llobregat, Barcelona, Spain
contact email	ssegarra@bioiberica.com
lab head
Joan Josep Bech-Serra
contact affiliation	Proteomics Unit - Josep Carreras Leukaemia Research Institute
contact email	jbech@carrerasresearch.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063531

PRIDE project URI

• PRIDE
  1. PXD063531
     1. Label: PRIDE project
     2. Name: Discovery, characterization and quantification of bioactive peptides contained in Palbio porcine intestinal mucosa hy-drolysate products.