PXD063500-1

PXD063500 is an original dataset announced via ProteomeXchange.

Title	Alpha-synuclein interacts with regulators of ATP homeostasis in mitochondria
Description	Mitochondrial dysfunction and accumulation of α-synuclein aggregates are hallmarks of the neurodegenerative Parkinson’s disease and may be interconnected. In order to investigate the interplay between α-synuclein and brain mitochondria at near atomic structural level we applied NMR and identified α-synuclein protein interactors using limited proteolysis-coupled mass spectrometry (LiP-MS). Several of the proteins identified were related to ATP synthesis and homeostasis and included subunits of ATP synthase and the adenylate kinase AK2. Furthermore, our data suggest that α-synuclein interacts with the Parkinson’s disease related protein DJ1. NMR analysis demonstrated that both AK2 and DJ1 bind to the C-terminus and other segments of α-synuclein. Using a functional assay for AK2, we showed that monomeric α-synuclein has an activating effect, whereas C-terminally truncated α-synuclein and α-synuclein in an amyloid fibrillar state had no significant effect on AK2 activity. Our results suggest that α-synuclein modulates ATP homeostasis in a manner dependent on its conformation and its C-terminal acidic segment.
HostingRepository	PRIDE
AnnounceDate	2025-07-19
AnnouncementXML	Submission_2025-07-18_20:00:50.181.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tetiana Serdiuk
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 07:14:55	ID requested
⏵ 1	2025-07-18 20:00:50	announced

Dataset with its publication pending

submitter keyword: LiP-MS, mitochondria,Alpha-synuclein

Paola Picotti
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich
contact email	picotti@imsb.biol.ethz.ch
lab head
Tetiana Serdiuk
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich, Switzerland
contact email	tserdiuk@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063500
     1. Label: PRIDE project
     2. Name: Alpha-synuclein interacts with regulators of ATP homeostasis in mitochondria