PXD063470 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multisite phosphorylation of intrinsically disordered region of Dishevelled |
| Description | When WNT binds to its receptor Frizzled (FZD) to activate the signaling, Dishevelled (DVL), the central component of Wnt signaling, is phosphorylated by redundant Casein kinase 1 (CK1) isoforms ε or δ, to transduce the signal downstream. Although the basic principles of Wnt signal transduction are known, the mechanistic aspects of DVL phosphorylation remain elusive. Here we identify Wnt-dependent multisite phosphorylation of a large and conserved serine/threonine (S/T) cluster within intrinsically disordered region (IDR), located between the PDZ and DEP domains. The detailed analysis of the phosphorylation and its effect on the flanking domains was performed with the construct containing both domains PDZ and DEP flanking the IDR (hDVL3 PDZ-DEP, aa 243-496). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-07 |
| AnnouncementXML | Submission_2026-04-07_00:02:12.547.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Functional Proteomics Platform FPP |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | monohydroxylated residue |
| Instrument | nanoACQUITY UPLC with HDX Technology; Synapt G2 HDMS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-29 23:55:32 | ID requested | |
| ⏵ 1 | 2026-04-07 00:02:13 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Dishevelled, phosphorylation, HDX-MS |
Contact List
| Cherine Bechara |
|---|
| contact affiliation | University of Montpellier, France |
| contact email | Cherine.bechara@umontpellier.fr |
| lab head | |
| Functional Proteomics Platform FPP |
|---|
| contact affiliation | FPP CNRS |
| contact email | contact@fpp.cnrs.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063470
- Label: PRIDE project
- Name: Multisite phosphorylation of intrinsically disordered region of Dishevelled
