PXD063444 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics reveals differential diagnosis biomarkers between sepsis and hemophagocytic syndrome |
| Description | Hemophagocytic Lymphohistiocytosis (HLH) shares many clinical features with sepsis. To improve HLH diagnosis and its differential diagnosis with sepsis, serum proteomic analyses of healthy donors, HLH and septic patients were performed. In this study, 24 individuals were enrolled in a label free MS/MS spectrometry analysis. Proteomic results revealed 537 differentially expressed proteins (DEP) between HLH and sepsis, 471 DEP between HLH and healthy donors and 37 DEP between sepsis and healthy donors. After protein identification and quantification, results were subjected to functional analysis to study impaired processes in each condition. These functional analyses showed that, apart from inflammation and lipid metabolism, the proteostasis network was deeply impaired in HLH condition. Considering this information, each protein fold changes, their function, and other previously published related to DEP, six proteins were validated by ELISA in a larger cohort of patients and healthy donors. Our results revealed that sCD300a, sCD300b and sCD25 could be specific serum biomarkers for HLH diagnosis. SAA-1 and LRG1 might be useful biomarkers for differential diagnosis between sepsis and HLH, and PSMB1 showed promising results for HLH specific and differential diagnosis. In this sense, PSMB1 was an interesting biomarker and its elevation in HLH patients may reflect an intensified demand for protein turnover, possibly driven by a higher activation of immunoproteasome. These insights contribute to expand our understanding of HLH pathophysiology in new pathways and highlight innovative therapeutic intervention such as Bortezomib and other next-generation inhibitors designed to modulate immunoproteasome activity |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-04_17:50:55.544.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mikel Azkargorta |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-29 06:24:32 | ID requested | |
| ⏵ 1 | 2026-01-04 17:50:56 | announced | |
Publication List
| Martin-Pestana D, Azkargorta M, Pilar-Orive FJ, Redondo S, Urrutia J, Calvo C, Elortza F, Astigarraga I, Garcia-Obregon S, Proteomics Reveals Differential Diagnosis Biomarkers Between Sepsis and Hemophagocytic Syndrome. Biomedicines, 13(12):(2025) [pubmed] |
| 10.3390/biomedicines13123113; |
Keyword List
| submitter keyword: hemophagocytic syndrome,Sepsis, label free |
Contact List
| Felix Elortza |
|---|
| contact affiliation | Proteomics Platform CIC bioGUNE Bizkaia Tech. Park Build. 800 Derio 48160 Spain |
| contact email | felortza@cicbiogune.es |
| lab head | |
| Mikel Azkargorta |
|---|
| contact affiliation | Proteomics Platform CIC bioGUNE |
| contact email | mazkargorta@cicbiogune.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063444 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063444
- Label: PRIDE project
- Name: Proteomics reveals differential diagnosis biomarkers between sepsis and hemophagocytic syndrome
