PXD063438 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Dynamic Proteomic Analysis of Human Liver Organoids: Optimal Maturation Stage for Drug Metabolism and Toxicology Applications |
| Description | Liver organoids have emerged as an innovative three-dimensional model system that effectively recapitulates the structural and functional complexities of human liver tissue, addressing critical limitations associated with traditional two-dimensional culture systems. This study presents a comprehensive temporal proteomic characterization of induced human liver organoids (iHLOs) by profiling 5,736 proteins across their four developmental stages Day 7, Day 15, Day 30, and Day 45 to identify optimal timepoints for drug metabolism and toxicological applications. The analysis revealed that Day 30 represents the peak of functional maturity, marked by the highest expression of key-metabolizing enzymes, such as cytochrome P450 3A4 (CYP3A4), along with cholangiocyte-specific proteins and hepatic metabolic enzymes. Functional validation of CYP3A4 activity through induction with rifampicin and inhibition with ketoconazole demonstrated comparable metabolic competence to existing in vitro liver models, including HepaRG cells and primary human hepatocytes. Interestingly, Day 45 organoids exhibited a shift toward a mesenchymal cell profile, with increased markers of hepatic stellate cells, suggesting utility in disease modeling applications. These findings suggest Day 30 of liver organoids as the optimal stage for drug metabolism and toxicological investigations, providing a proteomic framework that enhances the functional utility of iHLOs as physiologically relevant liver models. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-16 |
| AnnouncementXML | Submission_2026-01-16_09:30:19.171.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD063438 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jon (Jian-Jiang) Hao |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-29 04:12:47 | ID requested | |
| ⏵ 1 | 2026-01-16 09:30:19 | announced | |
Publication List
Keyword List
| submitter keyword: toxicology testing, hepatic Proteomics,Liver Organoids, Drug-Metabolizing Enzyme and Transporter profiles |
Contact List
| Menghang Xia |
|---|
| contact affiliation | National Center for Advancing Translational Sciences, National Institutes of Health, USA |
| contact email | mxia@mail.nih.gov |
| lab head | |
| Jon (Jian-Jiang) Hao |
|---|
| contact affiliation | Poochon Scientific |
| contact email | jon.hao@poochonscientific.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063438 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063438
- Label: PRIDE project
- Name: Dynamic Proteomic Analysis of Human Liver Organoids: Optimal Maturation Stage for Drug Metabolism and Toxicology Applications
