PXD063412-1

PXD063412 is an original dataset announced via ProteomeXchange.

Title	TRAF6 integrates innate immune signals to regulate glucose homeostasis via control of mitophagy
Description	Elevated or chronic innate immune activation plays a role in the progression of metabolic diseases such as diabetes, yet the influence of innate immune signaling on pancreatic β-cells is not well characterized. Here we report that TRAF6, an E3 ubiquitin ligase downstream of TLR signaling, maintains β-cell function during diet-induced obesity. Deletion or inhibition of TRAF6 impairs glucose homeostasis, mitochondrial function, and mitophagy during metabolic stress in both mouse and human islets. The combination of TRAF6 deficiency and palmitate exposure reduces localization of mitophagy machinery, such as BNIP3, to the mitochondria. Interestingly, co-deletion of Parkin restores glucose-stimulated insulin section, mitochondrial bioenergetics, and mitophagy. These findings suggest that Parkin-independent mitophagy mechanisms may be important for maintenance of β-cell function during metabolic stress.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_05:47:08.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yamei Deng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-28 19:38:00	ID requested
⏵ 1	2025-08-21 05:47:09	announced

Dataset with its publication pending

submitter keyword: Mitochondria, Innate Immunity, Beta cells, Metabolic Stress

Scott A. Soleimanpour
contact affiliation	Division of Metabolism, Endocriology & Diabetes and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48105, USA.
contact email	ssol@med.umich.edu
lab head
Yamei Deng
contact affiliation	University of Michigan
contact email	dengya@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063412

PRIDE project URI

• PRIDE
  1. PXD063412
     1. Label: PRIDE project
     2. Name: TRAF6 integrates innate immune signals to regulate glucose homeostasis via control of mitophagy