PXD063374-1

PXD063374 is an original dataset announced via ProteomeXchange.

Title	Tandem mass tag-based proteomics analysis to reveal growth stage-dependent pathways in Dendrobium nobile Lindl
Description	Objective: This study aimed to analyze differentially proteins at various growth stages of Dendrobium nobile Lindl and attempt to find some possible functional proteins related to plant growth or pharmacological activity. Methods: The samples were grouped into two categories based on their growth periods as 2-3 years (S1) and 7-8 years (S2), respectively. Tandem Mass Tag(TMT) labeling-based quantitative proteomics combined LC-MS/MS was applied to identify differential protein expression at various growth stages of Dendrobium nobile Lindl. Those proteins were analyzed by various bioinformatics methods. The activities of several antioxidant enzymes including superoxide dismutase(SOD), peroxidase (POD) and catalase (CAT) in the plant were specifically examined using commercially available assay kits. Results: A total of 6,032 proteins were identified with 283 differential proteins related to 168 up-regulated and 115 down-regulated. KEGG pathway enrichment analysis revealed that proteins involved in heat shock response, phenylpropanoid biosynthesis, and antioxidant activity showed significant changes across the growth stages. Specifically, a decrease in small heat shock proteins (sHSPs) was observed in older plants, potentially reducing their stress resilience, while proteins involved in lignin biosynthesis, such as SOD, CAT, and POD, were upregulated, suggesting improved stress response.
HostingRepository	iProX
AnnounceDate	2025-04-27
AnnouncementXML	Submission_2025-04-27_20:06:07.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cong Xie
SpeciesList	scientific name: Dendrobium nobile; NCBI TaxID: 94219;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-27 20:05:53	ID requested
⏵ 1	2025-04-27 20:06:07	announced

Dataset with its publication pending

submitter keyword: Dendrobium nobile Lindl, growth stage, Tandem mass tags (TMT), Proteomics, bioinformatics analysis

Wenying He
contact affiliation	Hainan Normal University
contact email	569130520@qq.com
lab head
Cong Xie
contact affiliation	Hainan Normal University
contact email	xc199345@outlook.com
dataset submitter

iProX dataset URI