PXD063310-1

PXD063310 is an original dataset announced via ProteomeXchange.

Title	Time-Resolved Proteomic Profiling of MRSA Under Sanguinarine Treatment
Description	This dataset contains time-resolved, label-free quantitative proteomic profiles of Staphylococcus aureus ATCC 43300 (MRSA) treated with the natural alkaloid sanguinarine. MRSA cultures were exposed to sanguinarine at the minimum inhibitory concentration (20 mg/L) and sampled at 2, 30, 60, and 120 minutes post-treatment. Untreated samples collected at identical time points served as controls. Proteins were extracted, digested using the FASP method, and analyzed by LC-MS/MS on a Q Exactive HF mass spectrometer. A total of 1,037 proteins were identified across all time points. This dataset supports the findings of a systems-level study exploring the antibacterial mechanism of sanguinarine, highlighting its impact on DNA repair, metabolic stress responses, and suppression of cell division and iron transport. Each condition was performed in three biological replicates.
HostingRepository	iProX
AnnounceDate	2025-04-25
AnnouncementXML	Submission_2025-04-25_00:46:37.549.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemeng Ji
SpeciesList	scientific name: Staphylococcus aureus PA; NCBI TaxID: 1046575;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-25 00:42:18	ID requested
⏵ 1	2025-04-25 00:46:37	announced

Dataset with its publication pending

submitter keyword: Sanguinarine, MRSA, Label-free quantitative proteomics, Time-resolved proteome, Antibacterial mechanism, Fuzzy C-means clustering, Oxidative stress, Cell division inhibition

Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	jixuemeng@nankai.edu.cn
lab head
Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	jixuemeng@nankai.edu.cn
dataset submitter

iProX dataset URI