PXD063271-1

PXD063271 is an original dataset announced via ProteomeXchange.

Title	Nuclear proteome analysis of mouse preimplantation embryos
Description	Mouse embryos at the 2-cell to 8-cell stages were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. Subsequently, embryos were split into single blastomeres, and nuclei were isolated from each blastomere using a micromanipulator. Proteomic samples were then prepared using the in situ digestion of alcohol-fixed cells (iSDAC) method with 50 (2-cell), 216 (4-cell), and 280 (8-cell) nuclei per sample. The nuclear proteome during mouse preimplantation development was identified by label-free quantification.
HostingRepository	jPOST
AnnounceDate	2026-04-24
AnnouncementXML	Submission_2026-04-23_08:00:17.316.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ayaka Kakihara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-24 00:37:33	ID requested
⏵ 1	2026-04-23 08:00:17	announced

Dataset with its publication pending

submitter keyword: preimplantation embryos, early development, developmental engineering, nuclear proteome

Masaki Matsumoto
lab head
Ayaka Kakihara
contact affiliation	Niigata University
dataset submitter

jPOST dataset URI

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