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PXD063264-1
PXD063264 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Trimodal probe for proteome profiling of AchE in animals |
| Description | Acetylcholinesterase (AChE) is a cholinergic enzyme that hydrolyzes esters of the neurotransmitter acetylcholine (Ach). Aberrant activity of AChE is closely associated with various neurodegenerative diseases and cancer. Current chemical probes for AChE detection mostly rely on fluorescence readout as a single-modality approach. The development of activity-based multimodal probes, which integrate the complementary advantages of near-infrared fluorescence (NIRF) imaging, photoacoustic (PA) detection, and proteome profiling, are therefore highly valuable. Such probes can provide more comprehensive information for deciphering the AChE code. Herein, we developed a set of multimodal probes, termed AFP1, AFP2, AFP3, and AFPP. AFP1/2/3, which enable NIRF/PA imaging, are capable of monitoring AChE activity in a continuous manner. AFPP is the first trimodal probe capable of NIRF/PA imaging and proteome profiling of AchE in live cells and animals. Notably, the usage of AFP2 and AFPP (1) highlights the superior capability of PA imaging for live animal studies; and (2) reveals significantly elevated AChE activity in microglia compared to astrocytes, as demonstrated in both cellular and murine models of depression. |
| HostingRepository | iProX |
| AnnounceDate | 2025-04-24 |
| AnnouncementXML | Submission_2026-01-21_22:45:57.052.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rui Wang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-04-23 22:07:50 | ID requested | |
| ⏵ 1 | 2026-01-21 22:45:57 | announced |
Publication List
| Xiang J, Wang H, Li L, Yang X, Xu G, Yan J, Li T, Shi Z, Xu C, Wang R, Tu Q, Wang M, Xie Y, Activity-Based Multimodal Probes Uncover the AChE-ADGRB Axis in Cellular and Murine Models of Depression. Anal Chem, 98(4):3040-3050(2026) [pubmed] |
Keyword List
| submitter keyword: AChE, Multimodal probes, Chemoproteome profiling |
Contact List
| Yusheng Xie | |
|---|---|
| contact affiliation | Shandong University |
| contact email | yushengxie@sdu.edu.cn |
| lab head | |
| Rui Wang | |
| contact affiliation | Shenzhen Bay Lab |
| contact email | wangrui@szbl.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
