PXD063260-1

PXD063260 is an original dataset announced via ProteomeXchange.

Title	Site-specific N-glycoproteomics of aging mouse uterus tissues for benchmarking the StrucGAP analysis platform
Description	This project presents the site-specific N-glycoproteomics dataset of aging mouse uterus tissues used to demonstrate and validate StrucGAP, a modular and scalable downstream analysis platform for glycoproteomics. Uterine samples were collected from 2- and 12-month-old C57BL/6 female mice (n = 15 per group), followed by protein extraction, tryptic digestion, TMT 10-plex labeling, intact glycopeptide enrichment, and LC-MS/MS analysis. A total of 20,251 unique intact glycopeptides (IGPs) were identified using the StrucGP search engine. The dataset captures detailed structural and quantitative glycosylation patterns across aging, enabling downstream analyses of glycan substructures, glycosylation site variation, and functional glycoproteomic annotation.
HostingRepository	iProX
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2026-03-23_02:25:04.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muyao Yang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	complex glycosylation; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-23 19:34:12	ID requested
⏵ 1	2026-03-23 02:25:05	announced

Yang M, Wu Y, Zhang Z, Xu Y, Lei T, Wang X, Jin Z, Hou K, Cai Y, Sun S, StrucGAP: a modular, streamlined and traceable data mining platform for structural and site-specific glycoproteomics. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: mouse uterus aging, data mining

Shisheng Sun
contact affiliation	Northwest University
contact email	suns@nwu.edu.cn
lab head
Muyao Yang
contact affiliation	Northwest University
contact email	Muyao_Yang@proton.me
dataset submitter

iProX dataset URI