PXD063245-1

PXD063245 is an original dataset announced via ProteomeXchange.

Title	Serum-derived human serum albumin as a source of undeclared proteins and compositional variability in commercially available IVF media
Description	This project explores the origin of undeclared proteins in commercially available IVF culture media using untargeted proteomic analysis (nanoLC-TIMS-TOF-MS/MS). Although human serum albumin (HSA) is the only declared protein component, we detected hundreds of additional proteins in unused media, indicating a non-standardized background that may affect embryo development and confound spent culture media (SCM) biomarker studies. We analyzed SCM samples from both successful and unsuccessful IVF embryo cultures, along with corresponding controls and blank media from multiple production lots and IVF cycles. Supplementation experiments demonstrated that adding plasma-derived HSA introduces variable protein contaminants, likely originating from human serum. These findings underscore the need for greater transparency and standardization in IVF media composition and highlight the sensitivity of proteomic workflows to background variability in embryo culture systems.
HostingRepository	PRIDE
AnnounceDate	2025-08-11
AnnouncementXML	Submission_2025-08-11_08:22:57.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Václav Pustka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-23 06:51:05	ID requested
⏵ 1	2025-08-11 08:22:57	announced

10.1007/S10815-025-03616-0;

submitter keyword: IVF media composition, proteomics, human serum albumin, spent culture medium, embryo culture

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Václav Pustka
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	vaclav.pustka@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD063245

PRIDE project URI

• PRIDE
  1. PXD063245
     1. Label: PRIDE project
     2. Name: Serum-derived human serum albumin as a source of undeclared proteins and compositional variability in commercially available IVF media