PXD063219-1

PXD063219 is an original dataset announced via ProteomeXchange.

Title	TAPBPR promotes editing of the HLA-B*44 peptide repertoire, increasing the presentation of peptides containing a C-terminal tryptophan
Description	Major histocompatibility complex class I (MHC-I) molecules play a key part in the adaptive immune response through the presentation of antigens to CD8+ T cells. The high degree of polymorphism in MHC-I leads to significant variation in their dependence on components of the antigen processing and presentation pathway such as TAP and tapasin, and their affinity for the peptide editor TAPBPR. Here, we investigated the influence of TAPBPR on the cell surface phenotype and peptide repertoire presented by two human leukocyte antigen (HLA) class I allotypes, HLA-B*44:02 and -B*44:05, which are known to differ drastically in their dependence on tapasin. While TAPBPR exhibits a reduced ability to bind to HLA-B molecules compared to HLA-A, we found that it could bind to both HLA-B*44:02 and -B*44:05. In contrast to tapasin depletion, loss of TAPBPR has a limited effect on cell surface expression of these two molecules. Analysis of the immunopeptidomes presented in the presence and absence of TAPBPR revealed while TAPBPR expression restricted the peptide repertoire presented on HLA-B*44:05, it diversified the repertoire presented on HLA-B*44:02. Overall, TAPBPR improved the predicted affinity of the peptides displayed on both the HLA-B*44 molecules. Furthermore, TAPBPR enhanced the presentation of peptides containing a C-terminal tryptophan residue. Our results show that TAPBPR can significantly impact the peptide repertoire of MHC-I molecules to which it binds weakly. Furthermore, this represents the first study which points to a role for TAPBPR in the selection of a specific peptide sequence on MHC class I molecules.
HostingRepository	PRIDE
AnnounceDate	2025-10-29
AnnouncementXML	Submission_2025-10-28_17:20:42.394.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Wacker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 23:09:34	ID requested
⏵ 1	2025-10-28 17:20:42	announced

Dataset with its publication pending

submitter keyword: None

Juliane Walz
contact affiliation	Department of Peptide-based Immunotherapy, Institute of Immunology, University and University Hospital Tübingen, Tübingen, Germany
contact email	juliane.walz@med.uni-tuebingen.de
lab head
Marcel Wacker
contact affiliation	Department of Peptide-based Immunotherapy, University and University Hospital Tübingen, Tübingen, Germany
contact email	marcel.wacker@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD063219

PRIDE project URI

• PRIDE
  1. PXD063219
     1. Label: PRIDE project
     2. Name: TAPBPR promotes editing of the HLA-B*44 peptide repertoire, increasing the presentation of peptides containing a C-terminal tryptophan