PXD063207-1

PXD063207 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of fava bean tissues: Optimization of extraction method and comparative analysis using LC-MS
Description	Fava beans (Vicia faba) have demonstrated potential to replace traditional animal-based protein sources. The quantity and quality of protein found in fava beans makes them a potential candidate for sustainable protein alternative. However, the understanding of their proteomic profile remains limited. In this study, we present an optimized proteomic workflow for fava beans, comparing the extraction efficiency of detergent-based vs chaotrope-based extraction methods. We conducted liquid chromatography-mass spectrometry (LC-MS) using data-independent acquisition (DIA) to improve coverage and analysed the protein profiles of different fava bean tissues, including dried mature beans, freeze dried fresh whole beans, outer coat, and cotyledon (core). Our results reveal significant differences in protein composition between extraction methods and bean tissues, providing insights into the most effective approach for studying fava bean proteomes. Future studies can utilize this optimized workflow for the functional characterization of fava bean proteins, advancing the exploration of sustainable plant-based protein sources. This study provides a valuable resource for advancing proteomic research of legume species, contributing to more efficient proteomic profiling in plant research.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:48:41.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Wilson
SpeciesList	scientific name: Vicia faba; NCBI TaxID: NEWT:3906;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 17:59:36	ID requested
⏵ 1	2026-06-28 16:48:42	announced

10.1016/J.JFCA.2026.109121;

submitter keyword: proteomics, legume proteomics, LC-MS profiling,Fava bean proteomics, Vicia faba

Dr Richard Wilson
contact affiliation	Proteomics facility, Central Science Laboratory, University of Tasmania
contact email	richard.wilson@utas.edu.au
lab head
Richard Wilson
contact affiliation	University of Tasmania
contact email	richard.wilson@utas.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063207

PRIDE project URI

• PRIDE
  1. PXD063207
     1. Label: PRIDE project
     2. Name: Proteomic profiling of fava bean tissues: Optimization of extraction method and comparative analysis using LC-MS