PXD063200 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity labeling of dense core vesicle protein VMAT |
| Description | Neuropeptide-containing dense core vesicles (DCVs) are generated in the neuronal cell body and circulate throughout the axonal arbor to supply distal release sites. This circulation depends on the anterograde kinesin-1 and kinesin-3 motors and the retrograde dynein-dynactin motor, however the adaptor proteins involved are poorly understood. In addition to their luminal neuropeptide cargo DCVs also contain specific transmembrane proteins including the vesicular monoamide transporter (VMAT). To identify potential components of DCV motor adaptor complexes in Drosophila neurons we performed proximity labeling based on a pan-neuronally expressed TurboID-VMAT chimera, as well as a free cytosolic TurboID used as a negative control. This approach may also shed light on the trafficking pathways involved in sorting VMAT and other membrane proteins to DCVs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-11_16:42:31.344.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michael Wierer |
| SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-22 09:55:37 | ID requested | |
| ⏵ 1 | 2026-01-11 16:42:32 | announced | |
Publication List
| Lund VK, Chirco A, Caliari M, Larsen AH, Tang KT, Gether U, Madsen KL, Wierer M, Kjaerulff O, A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles. J Cell Biol, 225(3):(2026) [pubmed] |
| 10.1083/jcb.202507071; |
Keyword List
| submitter keyword: synaptic vesicles, VMAT, neurons, axonal transport,Neuropeptide-containing dense core vesicles, Drosophila |
Contact List
| Ole Kjaerulff |
|---|
| contact affiliation | Department of Neuroscience, University of Copenhagen |
| contact email | okjaerulff@sund.ku.dk |
| lab head | |
| Michael Wierer |
|---|
| contact affiliation | Proteomics Research Infrastructure, University of Copenhagen |
| contact email | michael.wierer@sund.ku.dk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063200
- Label: PRIDE project
- Name: Proximity labeling of dense core vesicle protein VMAT
