PXD063196-1

PXD063196 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling of active and inactive neuronal Rab2 in flies
Description	Neuropeptide-containing dense core vesicles (DCVs) are generated in the neuronal cell body and circulate throughout the axonal arbor to supply distal release sites. This circulation depends on the anterograde kinesin-1 and kinesin-3 motors and the retrograde dynein-dynactin motor, however the adaptor proteins involved are poorly understood. Knock-out of the small GTPase Rab2 selectively disrupts retrograde DCV transport in Drosophila axons indicating that it is involved in regulation of dynein. To identify specific interactors of active neuronal Rab2 in flies, we performed proximity labeling based on a pan-neuronally expressed constitutively active GTP-locked TurboID-Rab2Q65L chimera, as well as an inactive GDP-locked TurboID-Rab2S20N variant.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_16:30:37.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 07:46:08	ID requested
⏵ 1	2026-01-11 16:30:38	announced

Lund VK, Chirco A, Caliari M, Larsen AH, Tang KT, Gether U, Madsen KL, Wierer M, Kjaerulff O, A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles. J Cell Biol, 225(3):(2026) [pubmed]

10.1083/jcb.202507071;

submitter keyword: neurons, axonal transport,Neuropeptide-containing dense core vesicles, Drosophila

Ole Kjaerulff
contact affiliation	Department of Neuroscience University of Copenhagen
contact email	okjaerulff@sund.ku.dk
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD063196

PRIDE project URI

• PRIDE
  1. PXD063196
     1. Label: PRIDE project
     2. Name: Proximity labeling of active and inactive neuronal Rab2 in flies