PXD063183-1

PXD063183 is an original dataset announced via ProteomeXchange.

Title	7-Octenoic Acid Exerts Anti-Fibrotic Effects on TGF-β1-Activated Hepatic Stellate Cells: Insights from Proteomic Analysis
Description	Liver fibrosis represents the liver’s adaptive response to sustained inflammation triggered by diverse insults such as dietary factors and infectious agents. Although early-stage fibrosis can be reversible, unchecked progression often leads to cirrhosis and hepatocellular carcinoma. Therapeutic options remain scarce, highlighting the urgent need for novel anti‑fibrotic compounds. In this study, we evaluated the in vitro anti‑fibrotic potential of 7‑octenoic acid (7‑OCT), a bioactive constituent identified from Moringa oleifera Lam. Human hepatic stellate LX‑2 cells were stimulated with TGF‑β1 (10 ng/mL) in the presence or absence of 7‑OCT. Fibrotic marker expression was quantified at both the mRNA and protein levels. Comprehensive proteomic profiling and subsequent in silico docking analyses were performed to identify putative molecular targets and clarify mechanisms of action. Our results demonstrate that 7‑OCT significantly suppresses the expression of α‑smooth muscle actin (α‑SMA), collagen type I, and matrix metalloproteinase‑9 (MMP‑9) by downregulating TGF‑β receptor 1 (TGFBR1) expression and inhibiting mothers against decapentaplegic homolog 3 (SMAD3) phosphorylation. Proteomic data further implicate focal adhesion pathway proteins in mediating these effects. Collectively, these preliminary findings suggest that 7‑OCT is a promising lead for the development of anti‑fibrotic therapies.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_22:15:50.619.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Watunyoo Buakaew
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 03:41:16	ID requested
⏵ 1	2025-11-09 22:15:51	announced

Dataset with its publication pending

submitter keyword: Hepatic stellate cell, 7-octenoic acid, Moringa oleifera Lam.,Liver fibrosis, Proteomics

Kanchana Usuwanthim
contact affiliation	Cellular and Molecular Immunology Research Unit (CMIRU), Faculty of Allied Health Science, Naresuan University, Phitsanulok, 65000, Thailand
contact email	kanchanau@nu.ac.th
lab head
Watunyoo Buakaew
contact affiliation	Department of Microbiology, Faculty of Medicine, Srinakharinwirot University
contact email	watunyoo@g.swu.ac.th
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD063183

PRIDE project URI

• PRIDE
  1. PXD063183
     1. Label: PRIDE project
     2. Name: 7-Octenoic Acid Exerts Anti-Fibrotic Effects on TGF-β1-Activated Hepatic Stellate Cells: Insights from Proteomic Analysis