PXD063170-1

PXD063170 is an original dataset announced via ProteomeXchange.

Title	H2S-mediated S-sulfhydration coordinates autophagy and infection of the rice blast fungus
Description	Hydrogen sulfide (H2S), a potent signaling molecule, regulates cellular activities in plants and mammals through S-sulfhydration, a post-translational modification. The role of H2S and its molecular targets in fungi, however, remain unclear. Our study demonstrates that H2S, synthesized by cystathionine γ-lyase (CSE1) in the rice blast fungus Magnaporthe oryzae, is essential for optimal fungal infection. Excessive H2S, through S-sulfhydration, impairs fungal infectivity by negatively regulating autophagy. Using quantitative proteomics, we identified numerous S-sulfhydrated proteins in M. oryzae, including the autophagy-related protein ATG18. We found that S-sulfhydration of a critical cysteine residue (Cys78) in ATG18 is essential for its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P), thereby maintaining the protein's structural stability and regulating autophagy. This study uncovers a new mechanism by which H2S-mediated S-sulfhydration controls autophagy in pathogenic fungi, and suggests the potential use of H2S donors as a strategy to control fungal diseases by targeting fungal development and infection structures, with broad implications for agriculture and disease management.
HostingRepository	PRIDE
AnnounceDate	2025-06-12
AnnouncementXML	Submission_2025-06-12_02:11:41.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hong Hu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-21 20:03:27	ID requested
⏵ 1	2025-06-12 02:11:42	announced

Dataset with its publication pending

submitter keyword: S-Sulfhydration；H2S；ATG18；Magnaporthe oryzae

Xiao-Lin Chen
contact affiliation	Huazhong Agricultural University
contact email	chenxiaolin@mail.hzau.edu.cn
lab head
hong Hu
contact affiliation	Huazhong Agricultural University
contact email	1073243798@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063170
     1. Label: PRIDE project
     2. Name: H2S-mediated S-sulfhydration coordinates autophagy and infection of the rice blast fungus