PXD063102-1

PXD063102 is an original dataset announced via ProteomeXchange.

Title	Cdk1 regulates cell-cell junction organization throughout the cell cycle to maintain epithelial barrier integrity
Description	Cdk1 is a master regulator of cell cycle progression and associated changes in cell shape, functions that have primarily been studied in single cells. However, cells exist within tissues, where cell cycle-associated shape changes must occur without compromising tissue function. As such, epithelial cells are tightly connected to form protective barriers, yet how epithelial cell-cell junctions are modulated throughout the cell cycle to maintain barrier function remains unclear. Here, we show that a pool of Cdk1 localizes to cell-cell contacts in epithelial cells and phosphorylates substrates at this cellular location throughout the cell cycle. Proteomic analysis identifies these Cdk1 substrates as components of cell-cell adhesion complexes. We demonstrate that Cdk1 activity controls the organization of cell-cell junctions and consequently is essential for preserving integrity of the epithelial barrier. These findings expand the role of Cdk1 to the regulation of cell-cell adhesion, establishing a direct link between the cell cycle machinery and epithelial integrity.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_02:42:59.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harmjan Vos
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: NEWT:9615;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-18 04:42:14	ID requested
⏵ 1	2026-06-12 02:42:59	announced

10.1126/SCISIGNAL.ADZ1593;

submitter keyword: Adherens junction, Epithelial barrier,Cdk1, Cell-cell adhesions, Cell cycle

Martijn Gloerich
contact affiliation	Center for Molecular Medicine University Medical Center Utrecht Utrecht 3584CG The Netherlands
contact email	M.Gloerich@umcutrecht.nl
lab head
Harmjan Vos
contact affiliation	University Medical Center Utrecht Center for Molecular Medicine
contact email	h.r.vos-3@umcutrecht.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063102

PRIDE project URI

• PRIDE
  1. PXD063102
     1. Label: PRIDE project
     2. Name: Cdk1 regulates cell-cell junction organization throughout the cell cycle to maintain epithelial barrier integrity