PXD063075 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human Retinal Organoid Model of Disease-Relevant Photoreceptor Cell Death Amenable to Drug Screening |
| Description | Dry age-related macular degeneration (AMD) is characterized by the progressive loss of retinal pigment epithelium cells in the macula, leading to photoreceptor degeneration and loss of central vision. Current treatments can only slow disease progression in some cases, but once photoreceptors are damaged, vision loss becomes irreversible. Therefore, there is an urgent need to develop therapies that prevent photoreceptor cell death. A critical step toward this goal is establishing pathophysiologically relevant human disease models for therapeutic testing. In this study, we developed a human- induced pluripotent stem cell-derived retinal organoid (RO) model that recapitulates key aspects of AMD-associated photoreceptor degeneration. Since cigarette smoking is a major environmental risk factor for AMD, we exposed ROs to cigarette smoke extract (CSE) to induce cellular stress. CSE treatment resulted in increased reactive oxygen species, mitochondrial membrane depolarization, and cell death primarily in the outer nuclear layer. Photoreceptor degeneration in this model involves the activation of the intrinsic apoptotic pathway and ferroptosis, which is accompanied by lipid peroxidation and dysregulation of the glutathione system. Comprehensive proteomic analysis confirmed the dysregulation of metabolic and stress response pathways. The integration of this model with robust, quantitative outcome measures in live ROs offers a powerful platform for preclinical drug screening and therapeutic evaluation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-14 |
| AnnouncementXML | Submission_2026-04-14_06:22:09.548.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Anthony Saviola |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-17 10:34:47 | ID requested | |
| ⏵ 1 | 2026-04-14 06:22:10 | announced | |
Publication List
Keyword List
Contact List
| Natalia Vergara |
|---|
| contact affiliation | University of Colorado Anschutz Medical Campus |
| contact email | NATALIA.VERGARA@CUANSCHUTZ.EDU |
| lab head | |
| Anthony Saviola |
|---|
| contact affiliation | University of Colorado Anschutz Medical Campus |
| contact email | anthony.saviola@cuanschutz.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD063075 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063075
- Label: PRIDE project
- Name: Human Retinal Organoid Model of Disease-Relevant Photoreceptor Cell Death Amenable to Drug Screening
