PXD063057-1

PXD063057 is an original dataset announced via ProteomeXchange.

Title	MARCH1 ubiquitination of MHC II generates a branched K63/K11 ubiquitin chain.
Description	Major histocompatibility class II (MHC II) is critical for adaptive immunity. MHC II intracellular trafficking and degradation is regulated by ubiquitination, with poly-ubiquitination (Ub) of MHC II directing it away from the plasma membrane. The MHC II poly-Ub chain that dictates the fate and function of ubiquitinated MHC II has not been characterized. Here, we describe the poly-Ub chain associated with MHC II in primary dendritic cells (DCs) and B cells. Analysis was conducted for endogenous H2-A and H2-E molecules immunoprecipitated from primary cells. We show that the ubiquitination of both I-A and I-E expressed by DCs and B cells is dependent on the E3 Ub ligase MARCH1. Comprehensive Ub chain analysis reveals MARCH1-dependent poly-Ub associated with MHC II is composed of Ub chains with branched K11/K63 linkages.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-12-01_01:29:10.415.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-17 01:28:18	ID requested
⏵ 1	2025-12-01 01:29:11	announced

10.1038/s41598-025-25817-4;

Liu H, Francis L, Dagley LF, Cobbold SA, Webb AI, Komander D, Villadangos JA, Mintern JD, Major histocompatibility class II in murine antigen presenting cells is modified with a branched K63 and K11-linked ubiquitin chain. Sci Rep, 15(1):41884(2025) [pubmed]

submitter keyword: Ubiquitin, MHCII, Ub-code, AQUA peptides

Prof. Justine D. Mintern
contact affiliation	Biochemistry and Pharmacology, MDHS Associate Dean of Graduate Research Bio21 Institute The University of Melbourne, Victoria 3010 Australia
contact email	jmintern@unimelb.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD063057

PRIDE project URI

• PRIDE
  1. PXD063057
     1. Label: PRIDE project
     2. Name: MARCH1 ubiquitination of MHC II generates a branched K63/K11 ubiquitin chain.