PXD062994 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sulfur Starvation Induces an Fe-Replete Response and Attenuates Virulence Pathways in Pseudomonas aeruginosa PAO1 |
| Description | Specific bacteria grown with alternative sulfur sources leads to metabolic reprogramming that includes the expression of sulfur-scavenging and antioxidant genes. To determine the detailed metabolic effect of sulfur starvation, P. aeruginosa PAO1 was grown in sulfur-free media alone and with the addition of sulfate and evaluated by label-free proteomics. Proteomics analyses identified 729 differentially expressed proteins from 2633 confidently identified proteins. Differentially expressed features were analyzed for pathway enrichment using STRING (proteomics),leveraging Gene Ontology and KEGG annotations. corresponding proteins involved in sulfur assimilation and metabolism, including those of the msu, ssu, and cys operons, showed an increased abundance during sulfur starvation. Sulfur starvation depressed Fe-uptake gene/pathway expression, including the pyoverdine (pvd), pyochelin (pch), and heme-uptake (phu and has) operons. Expression of genes from virulence pathways was also diminished, including quorum sensing, MDR efflux pumps, phenazine biosynthetic genes, and hydrolytic enzymes thought to be regulated by the ferric uptake regulator and PrrF sRNAs. In contrast, there was a significant increase in expression of proteins involved in Fe-storage, including bfrB, dps, and PA4880. Our study demonstrates that sulfur starvation leads to an Fe-replete response in P. aeruginosa PAO1 due to a disruption in Fe homeostasis and uncovered the suppression of critical virulence gene products during sulfur starvation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:16:30.126.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tonya Zeczycki |
| SpeciesList | scientific name: Pseudomonas aeruginosa ATCC 700888; NCBI TaxID: NEWT:1163394; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-15 10:40:50 | ID requested | |
| ⏵ 1 | 2025-11-09 16:16:31 | announced | |
Publication List
| 10.1186/s12866-025-04442-1; |
| Ugochukwu CG, Schwartz TS, Zeczycki TN, Goodwin DC, Ellis HR, Sulfur starvation induces an Fe-replete response and attenuates virulence pathways in Pseudomonas aeruginosa PAO1. BMC Microbiol, 25(1):708(2025) [pubmed] |
Keyword List
| submitter keyword: sulfur,Pseudomonas aeruginosa, oxidative stress, Fe-Replete, Virulence Pathways |
Contact List
| Holly R. Ellis |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, NC, USA |
| contact email | ellish20@ecu.edu |
| lab head | |
| Tonya Zeczycki |
|---|
| contact affiliation | Brody School of Medicine at ECU |
| contact email | zeczyckit@ecu.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062994
- Label: PRIDE project
- Name: Sulfur Starvation Induces an Fe-Replete Response and Attenuates Virulence Pathways in Pseudomonas aeruginosa PAO1
