PXD062991-1

PXD062991 is an original dataset announced via ProteomeXchange.

Title	Mycotoxin-driven proteome remodeling of Triticum aestivum defines host responses and pathogen adaptation to emerging threats
Description	Fusarium head blight (FHB) is a devastating fungal disease of cereal crops causing substantial reductions in grain quality and yield, impacting Canada’s economy. Fusarium graminearum, the causative agent of FHB in wheat, also produces harmful mycotoxins, including deoxynivalenol (DON), 3-acetyl DON (3ADON), 15-acetyl DON (15ADON), and nivalenol (NIV), which impact livestock and human health upon consumption of contaminated grains in feed and processed foods, respectively. Critically, over the past 15 years, surveillance programs have defined the synthesis of a novel trichothecene, 3ANX, and the deacetylated variant, NX, with increased virulence compared to DON. In this study, we assessed the impact of 3ANX from dual proteome perspectives of wheat and fungi to identify proteins and pathways activated in response to the emerging chemotype. We defined a core wheat proteome detected across all isolates (15ADON- and 15ADON+3ANX-producing, and untreated controls), and explored changes in protein abundance associated with defense response, seed storage and grain development, and reduced photosynthesis upon infection. Conversely, we identified 32 wheat proteins produced only in the presence of 15ADON+3ANX isolates, providing further insight into chemotype-specific responses of wheat. Assessment from the fungal perspective reported 119 proteins exclusive to the 15ADON+3ANX isolates, including those associated with virulence and mycotoxin production. Lastly, investigation of isolate-specific proteome changes showed a significant reduction in mycotoxin protective mechanisms in wheat upon exposure to two 15ADON+3ANX isolates, as well as a novel connection between elevated ergosterol biosynthesis and 3ANX production in F. graminearum. Together, we our study characterizes distinct protein production profiles in wheat and F. graminearum in response to 3ANX treatment and provides evidence that these molecular changes influence fungal virulence and host defense responses
HostingRepository	PRIDE
AnnounceDate	2026-01-09
AnnouncementXML	Submission_2026-01-09_08:42:52.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seyedehsanaz Ramezanpour
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: NEWT:4565;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-15 09:52:34	ID requested
⏵ 1	2026-01-09 08:42:53	announced

10.1038/S44383-025-00020-6;

submitter keyword: NX toxin, reactive oxygen species, Fusarium graminearum, Proteomics,Wheat, Mycotoxins, Deoxynivalenol

Jennifer Geddes-McAliste
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Seyedehsanaz Ramezanpour
contact affiliation	Molecular and Cellular Biology Department, University of Guelph
contact email	ramezanp@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD062991

PRIDE project URI

• PRIDE
  1. PXD062991
     1. Label: PRIDE project
     2. Name: Mycotoxin-driven proteome remodeling of Triticum aestivum defines host responses and pathogen adaptation to emerging threats