PXD062961-1

PXD062961 is an original dataset announced via ProteomeXchange.

Title	HNF4α contributes to hepatic CAR dysfunction in polymicrobial sepsis
Description	Constitutive androstane receptor (CAR) is a xenobiotic nuclear receptor mainly expressed in the liver, where it regulates drug metabolism and energy homeostasis. CAR has emerged as a promising therapeutic target for diabetes, fatty liver disease, and alcoholic liver disease, but it has not been investigated in the context of sepsis. Here, we show that sepsis impairs CAR function in the liver, partly due to reduced transcription mediated by HNF4α, the key liver identity transcription factor, and partly due to decreased DNA binding, caused by chromatin remodeling, also mediated by HNF4α. This impairment leads to the downregulation of several genes and proteins involved in monocarboxylic acid, fatty acid, and xenobiotic metabolism, contributing to increased sepsis lethality, and is associated with an elevated hepatic acute phase response. LC-MSMS analysis was used to identify the proteins differentially expressed in the liver 24h after sepsis onset.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:20:52.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Dufour
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-14 23:41:52	ID requested
⏵ 1	2025-09-14 16:20:53	announced

10.3389/fimmu.2025.1625104;

Van Dender C, Timmermans S, Roes M, Hellemans M, Moens E, Nuyttens L, Claes M, Roman B, De Bosscher K, Vandewalle J, Libert C, contributes to hepatic CAR dysfunction in polymicrobial sepsis. Front Immunol, 16():1625104(2025) [pubmed]

submitter keyword: LC-MSMS, CLP-induced peritoneal sepsis,Mouse, Liver

Claude Libert
contact affiliation	Center for Inflammation Research, VIB, Ghent, Belgium
contact email	claude.libert@irc.vib-ugent.be
lab head
Sara Dufour
contact affiliation	VIB-UGent
contact email	sara.dufour@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062961

PRIDE project URI

• PRIDE
  1. PXD062961
     1. Label: PRIDE project
     2. Name: HNF4α contributes to hepatic CAR dysfunction in polymicrobial sepsis