PXD062939-1

PXD062939 is an original dataset announced via ProteomeXchange.

Title	Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integrity
Description	Aggressive brain tumors often exhibit immunologically ‘cold’ microenvironment, where the vascular barrier impedes effective immunotherapy in poorly understood ways. Tumor vasculature also plays a pivotal role in immunoregulation and antitumor immunity. Here we show that small GTPase Rab27 controls the vascular morphogenesis and permeability for blood content and immune effectors. Thus, in Rab27a/b double knock out (Rab27-dKO) mice, the brain vasculature is abnormally scarce while the blood vessels become dysmorphic and hyperpermeable in the context of brain tumors, including syngeneic glioblastoma. These defects are reflected in rearrangements of endothelial cell subpopulations with underlying diminution of venous endothelial subtype along with changes in gene and protein expression. Notably, Rab27-dKO brain endothelial cells exhibit deficient tight junctions, whereby they enable large-scale extravasation of cytotoxic T cells into the tumor mass. We show that Rab27-regulated vascular T cell infiltration can be exploited to enhance adoptive T cell therapy in syngeneic brain tumors.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-01_16:13:48.081.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lata Adnani
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-14 09:23:38	ID requested
⏵ 1	2025-06-01 16:13:48	announced

Adnani L, Meehan B, Kim M, Choi D, Rudd CE, Riazalhosseini Y, Rak J, Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integrity. Sci Adv, 11(21):eadr6940(2025) [pubmed]

10.1126/sciadv.adr6940;

submitter keyword: Brain tumor, adoptive immunity, cytotoxic T-cells, glioma, vasculature, blood brain barrier, endothelial cells, tight junctions., tumor microenvironment

Janusz Rak
contact affiliation	Jack Cole Chair in Pediatric Hematology/Oncology Professor, Department of Pediatrics McGill University
contact email	janusz.rak@mcgill.ca
lab head
Lata Adnani
contact affiliation	Research Institute of McGill University Health Center
contact email	lata.adnani@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD062939

PRIDE project URI

• PRIDE
  1. PXD062939
     1. Label: PRIDE project
     2. Name: Immune cell infiltration into brain tumor microenvironment is mediated by Rab27-regulated vascular wall integrity