PXD062930-1

PXD062930 is an original dataset announced via ProteomeXchange.

Title	Storage Protein-Mediated Translation Control Links Juvenile Diet to Longevity in Drosophila
Description	Dietary restriction (DR), whether applied during adulthood or juvenile stages, extends lifespan across diverse species. However, the mechanisms by which early-life dietary interventions influence adult physiology and longevity remain poorly understood. Here, using Drosophila as a model, we demonstrate that protein restriction during the larval stage (early-life protein restriction, ePR) promotes adult lifespan by reducing storage protein levels. Stable isotope tracing reveals that dietary amino acids obtained in the larval stage are retained into adulthood, especially incorporated into ribosomal proteins. This is mediated by larval serum protein 2 (Lsp2), a major storage protein, whose expression is durably downregulated by ePR. Both dietary (ePR) and genetic (Lsp2-RNAi) reduction of the protein storage lead to decreased ribosomal protein levels and translation activity in early adulthood. Notably, these storage proteins are enriched in aromatic amino acids such as tyrosine, and larval tyrosine restriction alone is sufficient to suppress translation and promote longevity. These findings show that storage proteins mediate the effect of larval nutrition on adult longevity via controlling translation. Our study uncovers a previously unrecognised mechanism of nutritional memory that links early-life diet to life-long organismal health.
HostingRepository	jPOST
AnnounceDate	2026-07-17
AnnouncementXML	Submission_2026-07-16_16:27:40.566.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification; Label:13C(6)15N(2); Label:13C(6)15N(4); 6x(13)C labeled L-arginine; unknown modification
Instrument	Q Exactive Plus; asymmetric track lossless time-of-flight analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-14 05:34:47	ID requested
⏵ 1	2026-07-16 16:27:41	announced

Dataset with its publication pending

submitter keyword: pulse SILAC, fly, early-life protein restriction (ePR)

Koshi Imami
lab head
Koshi Imami
contact affiliation	RIKEN
dataset submitter

jPOST dataset URI

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