PXD062917 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Post-gastrulation amnioids (PGAs) as a stem cell-derived model of week 2-4 of human extra-embryonic development |
| Description | The amnion, an essential extra-embryonic tissue in mammalian embryos, is thought to provide crucial signaling, structural and nutritional support during pregnancy. Despite its pivotal importance, studying primate amnion formation and function has been hampered by the lack of accurate in-vitro models. Here, we present a human embryonic stem cell-derived 3D model of the post-gastrulation amnion (PGA) that faithfully recapitulates extra-embryonic development up to 4-weeks post-fertilization closely mimicking the functional traits of the human amniotic sac. PGAs self-organize forming the amnion and the yolk sac and are surrounded by extra-embryonic mesoderm, which forms a connecting stalk. Using PGAs, we show that GATA3 is required and sufficient for amniogenesis and reveal an autoregulatory feedback loop governing amnion formation, whereby extra-embryonic signals promote amnion specification. The reproducibility and scalability of the PGA system, with its precise cellular composition and structural integrity, opens new avenues for investigating embryo-amnion interactions beyond gastrulation and offers an ideal platform for large-scalegenetic, pharmacological, and biomechanical studies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-19 |
| AnnouncementXML | Submission_2026-01-19_09:16:21.022.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mark Skehel |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-14 01:57:18 | ID requested | |
| ⏵ 1 | 2026-01-19 09:16:21 | announced | |
Publication List
Keyword List
| submitter keyword: development,Stem cell, aminoids, lc-ms/ms |
Contact List
| Silvia D. M. Santos |
|---|
| contact affiliation | Quantitative Stem Cell Biology Lab The Francis Crick Institute 1 Midland Road London NW1 1AT |
| contact email | silvia.santos@crick.ac.uk |
| lab head | |
| Mark Skehel |
|---|
| contact affiliation | Francis Crick Institute |
| contact email | skehelm@crick.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD062917 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062917
- Label: PRIDE project
- Name: Post-gastrulation amnioids (PGAs) as a stem cell-derived model of week 2-4 of human extra-embryonic development
