PXD062902-1

PXD062902 is an original dataset announced via ProteomeXchange.

Title	Identification of GSDME-interacting proteins in HK-2 cells by immunoprecipitation-coupled LC-MS/MS analysis
Description	This project aims to identify GSDME-associated proteins in human renal tubular epithelial (HK-2) cells using immunoprecipitation followed by LC-MS/MS. HK-2 cells overexpressing Flag-tagged GSDME were lysed, and protein complexes were enriched using anti-Flag antibody and Protein A/G magnetic beads. Protein lysates were analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was performed using the UniProtKB human database. The dataset provides insights into the potential protein interactors of GSDME and their relevance to post-translational regulation.
HostingRepository	iProX
AnnounceDate	2025-04-12
AnnouncementXML	Submission_2025-08-10_21:19:07.684.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pengxiao Sun
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-13 21:54:58	ID requested
⏵ 1	2025-08-10 21:19:08	announced

Chen Q, Sun P, Zhou J, Long T, Xiao A, Liu Z, Xu S, Lei W, Zhang R, Tian J, Zhou M, Hu Z, Zhu F, Nie J, Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice. Nat Commun, 16(1):6807(2025) [pubmed]

submitter keyword: GSDME, LC-MS/MS, immunoprecipitation, HK-2 cells, protein interaction

Jing Nie
contact affiliation	Division of Nephrology, Nanfang Hospital, Southern Medical University
contact email	Niejing@smu.edu.cn
lab head
Pengxiao Sun
contact affiliation	Division of Nephrology, Nanfang Hospital, Southern Medical University
contact email	pxsun1995@163.com
dataset submitter

iProX dataset URI