PXD062852-1

PXD062852 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The E3 ubiquitin ligase SPRYD3-MYCBP2(PAM) regulates mitotic cell fate and ubiquitination of USP11 to control spindle assembly
Description	We show that the atypical E3 ligase MYCBP2 binds to the SPRY-domain containing protein SPRYD3 to form a new E3 ubiquitin ligase complex. We identified the DUB USP11 as the first substrate of MYCBP2/SPRYD3. Interestingly, USP11 is ubiquitinated by MYCBP2/SPRYD3 on a cysteine residue, C318, leading to proper bipolar spindle formation and mitotic slippage in cells treated with microtubule (MT) poisons.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_09:44:21.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandra Rita Turi da Fonte Dias
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-11 06:41:17	ID requested
⏵ 1	2025-10-22 09:44:22	announced

Publication List

10.1016/J.JBC.2025.110785;

10.1016/J.JBC.2025.110785;

Keyword List

submitter keyword: ubiquitylation, mitotic slippage,MYCBP2, deubiquitinase, E3 ligase, USP11

submitter keyword: ubiquitylation, mitotic slippage,MYCBP2, deubiquitinase, E3 ligase, USP11

Contact List

Ingrid Hoffmann
contact affiliation	D345, Cell Cycle and Carcinogenesis, Deutsches Krebsforschungszentrum Heidelberg
contact email	ingrid.hoffmann@dkfz-heidelberg.de
lab head
Alexandra Rita Turi da Fonte Dias
contact affiliation	Deutsches Krebsforschungszentrum (DKFZ) Heidelberg
contact email	alexandrarita.turidafontedias@dkfz-heidelberg.de
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062852
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062852

PRIDE project URI

Repository Record List

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