PXD062825-1

PXD062825 is an original dataset announced via ProteomeXchange.

Title	Near atomic structure of the PorKN rings, disulfide bonded to PorG and bound to Attachment Complexes, provides mechanistic insights into the type IX secretion system: CE and Co-immunoprecipitation
Description	The Type IX Secretion System exports proteins across the outer membrane (OM) of bacteria in the Bacteroidota phylum, however, the mechanistic details remain unknown. Here, we present a ~3.5Å cryo-EM structure of the periplasmic rings comprising 32-33 subunits each of PorK and PorN. Additionally, we show the presence of a critical disulfide bond between PorK and the PorG OM protein that is essential for protein secretion and demonstrate that the Attachment Complexes bind to and are localized above the PorKN rings. Overall, each ring resembles a cogwheel with PorN forming cog-like projections on the periplasmic side and the flat surface of PorK orienting towards the OM. Given these results, we propose that the PorLM motor drives the rotation of the PorKN cogwheel together with PorG and associated Attachment Complexes, potentially providing the energy to complete protein secretion and the coordinated cell surface attachment of the secreted cargo.
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_18:24:25.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhana Gorasia
SpeciesList	scientific name: Porphyromonas gingivalis (strain ATCC BAA-308 / W83); NCBI TaxID: 242619; scientific name: Porphyromonas gingivalis ATCC 33277; NCBI TaxID: 431947;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 17:16:20	ID requested
⏵ 1	2025-07-20 18:24:26	announced

Dataset with its publication pending

submitter keyword: Porphyromonas gingivalis, type IX secretion system

Eric Reynolds
contact affiliation	The University of Melbourne, Melbourne Dental School, Australia
contact email	e.reynolds@unimelb.edu.au
lab head
Dhana Gorasia
contact affiliation	The University of Melbourne
contact email	gorasiad@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD062825

PRIDE project URI

• PRIDE
  1. PXD062825
     1. Label: PRIDE project
     2. Name: Near atomic structure of the PorKN rings, disulfide bonded to PorG and bound to Attachment Complexes, provides mechanistic insights into the type IX secretion system: CE and Co-immunoprecipitation