PXD062822-1

PXD062822 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profile of Human Benign and Malignant Adrenocortical Neoplasia.
Description	The molecular pathogenesis of adrenocortical diseases, particularly adrenocortical carcinoma (ACC), has advanced considerably in recent years due to the application of high-throughput molecular analysis. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers an approach for identifying and quantifying numerous proteins in biological samples. Aiming to identify differences in adrenal cortex disease protein signatures that may contribute to understanding their distinct pathogenesis, we used LC-MS/MS and bioinformatics analysis to compare the proteomic profiles of normal human adrenal samples (NHA), adrenocortical adenomas (ACA), adrenocortical carcinomas (ACC), and primary macronodular adrenocortical hyperplasia (PMAH) tumor samples, with (w) and without (wt) ARMC5 mutation. A total of 7,350 proteins were identified, with 3,976 proteins quantified in all analyzed samples, and differentially expressed proteins (DEPs) noted as upregulated and downregulated were highlighted as relevant. We identified 27 DEPs in ACA vs. NHA, where MT3 and RBM3 are upregulated, and NEFL, HAAO, SOD3, IGHA2, and HDGFL3 are the top five of 25 downregulated proteins; 49 DEPs in ACC vs. NHA, with DEFA3, NUP160, SRRM2, and RBM3 upregulated, alongside SOD3, CNRIP, DNAH1, GAMT, ADIRF, and CA3 as the top six of 45 downregulated proteins; 81 DEPs in PMAH vs. NHA, with RBM3, RIGI, SPAG9, and SRRM2 proteins as the top four of 9 upregulated proteins, and 72 downregulated proteins with AFM, CA 3, IGKV1-5, ADIRF, and LRG1 as the top five; ACC vs. ACA identified 64 upregulated and 48 downregulated DEPs, with PHGDH (SERA), PDCD4, MAGED2, ERAP2, FTO, DUSP23, UBLCP 1, NSUN2, TP53BP1, NUP107 as the top ten upregulated, and RMDN2, CSRP1, ACAD11, PACSIN3, HAGH (GLO2), ALG5, NEXN, ABLIM1, LBP, and SYNPO2 as the top ten downregulated in ACC. DEPs analysis in PMAHw vs. PMAHwt showed the lowest number of DEPs, with NOSTRIN, PCP4, C4 BPB, and SERB (PSPH) as the top four of 12 upregulated proteins, and 4 downregulated proteins EST2 (TERT), PNPT1, RDH13, and STAB1 in PMAHw. Comparative analysis highlighted several targets for functional analysis and validation to determine the mechanism of action and protein biomarkers.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:25:08.273.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062822
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean Lucas Kremer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-10 12:52:32	ID requested
⏵ 1	2025-12-14 16:25:09	announced

Kremer JL, Ortega HS, Siqueira-Souza T, Angeli CB, Iwai LK, Lotfi CFP, Comparative Proteomic Profiling of Adrenocortical Neoplasia Using Mass Spectrometry. J Proteome Res, 24(12):6186-6202(2025) [pubmed]

10.1021/acs.jproteome.5c00740;

10.6019/PXD062822;

submitter keyword: Human adrenal gland, adrenocortical tumors, adrenocortical carcinoma, normal adrenal cortex, adrenocortical adenona, primary macronodular adrenocortical hyperplasia

Jean Lucas
contact affiliation	Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo,São Paulo, Brazil, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP, 05508-000.
contact email	jl.kremer@usp.br
lab head
Jean Lucas Kremer
contact affiliation	Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science, University of São Paulo
contact email	jl.kremer@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD062822

PRIDE project URI

• PRIDE
  1. PXD062822
     1. Label: PRIDE project
     2. Name: Proteomic Profile of Human Benign and Malignant Adrenocortical Neoplasia.