PXD062800-1

PXD062800 is an original dataset announced via ProteomeXchange.

Title	Near atomic structure of the PorKN rings, disulfie bonded to PorG and bound to the Attachment Complexes, provide mechanistic insights into the Type IX Secretion System
Description	The Type IX Secretion System exports proteins across the outer membrane (OM) of bacteria in the Bacteroidota phylum, however, the mechanistic details remain unknown. Here, we present a 3.5 angstrom cryo-EM structure of the periplasmic rings comprising 32-33 subunits each of PorK and PorN. Additionally, we show the presence of a critical disulfide bond between PorK and the PorG OM protein that is essential for protein secretion and demonstrate that the Attachment Complexes bind to and are localized above the PorKN rings. Overall, each ring resembles a cogwheel with PorN forming cog-like projections on the periplasmic side and the flat surface of PorK orienting towards the OM. Given these results, we propose that the PorLM motor drives the rotation of the PorKN cogwheel together with PorG and associated Attachment Complexes, potentially providing the energy to complete protein secretion and the coordinated cell surface attachment of the secreted cargo.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:05:52.722.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062800
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Veith
SpeciesList	scientific name: Porphyromonas gingivalis W50; NCBI TaxID: 1125722;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-09 22:58:04	ID requested
⏵ 1	2025-08-24 16:05:53	announced

10.1038/s41467-025-63163-1;

10.6019/PXD062800;

Gorasia DG, Hanssen E, Mudaliyar M, Morton CJ, Valimehr S, Seers C, Zhang L, Doyle MT, Ghosal D, Veith PD, Reynolds EC, Insights into type IX secretion from PorKN cogwheel structure bound to PorG and attachment complexes. Nat Commun, 16(1):7735(2025) [pubmed]

submitter keyword: type 9 secretion system,porphyromonas gingivalis

Eric Charles Reynolds
contact affiliation	Melbourne Dental School
contact email	e.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062800
     1. Label: PRIDE project
     2. Name: Near atomic structure of the PorKN rings, disulfie bonded to PorG and bound to the Attachment Complexes, provide mechanistic insights into the Type IX Secretion System