PXD062798 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bioengineered 3D human heart valve tissues with enhanced maturational properties for disease modelling and therapeutic applications |
| Description | Therapeutic management of heart valve disease is currently hampered by a lack of mechanistic understanding of human valve development and pathobiology. Here, we develop a protocol to generate 3-dimensional human heart valve tissues from pluripotent stem cells with enhanced maturational properties closely mirroring the valve interstitial cell and extracellular matrix profile of native heart valves. Importantly, bioengineered heart valves transplanted subcutaneously in rats did not calcify, highlighting potential clinical utility as a cellular therapy. In parallel, using a high-throughput 96-well platform, we demonstrate that bioengineered human heart valves can be used for modelling valve disease. For example, treatment with inflammatory cytokines augmented tissue passive tension and upregulated molecular markers associated with valve calcification and fibrosis. Thus, bioengineered human heart valve tissues provide a platform to understand human heart valve development, disease pathogenesis and as a potential regenerative therapy for heart valve disease. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-16 |
| AnnouncementXML | Submission_2026-08-15_17:27:07.269.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sean Humphrey |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-09 22:17:32 | ID requested | |
| ⏵ 1 | 2026-08-15 17:27:08 | announced | |
Publication List
| He Y, Jalili A, Jones CB, He H, Beni L, Lamont D, Hu J, Yost M, Davidson LA, Zheng SY, Li G, Human iPSC-derived heart valve-like assembloids model valve development and disease pathology. Cell Stem Cell, 33(9):1514-1527.e9(2026) [pubmed] |
| 10.1016/j.stem.2026.07.011; |
Keyword List
| submitter keyword: heart valve, iPSC, iPSCs, cardiomyocyte, maturation,human |
Contact List
| Sean Humphrey |
|---|
| contact affiliation | Murdoch Children's Research Institute, Melbourne, Australia |
| contact email | sean.humphrey@mcri.edu.au |
| lab head | |
| Sean Humphrey |
|---|
| contact affiliation | Murdoch Children's Research Institute |
| contact email | sean.j.humphrey@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD062798 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062798
- Label: PRIDE project
- Name: Bioengineered 3D human heart valve tissues with enhanced maturational properties for disease modelling and therapeutic applications
