⮝ Full datasets listing
PXD062788-1
PXD062788 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling of primary cilia in the developing brain uncovers new regulators of cortical development |
| Description | In the developing brain, the primary cilia of radial glial cells extend from the surface of the lateral ventricle, serving as the signaling hub to integrate environmental cues critical for brain formation. Dysfunctions in ciliary proteins contribute to a wide range of brain structural abnormalities in ciliopathies, yet identifying the bona fide ciliary protein components in the brain remains a significant challenge. Here, using proximity labeling and quantitative proteomics, we systematically charted proteins localized to the cilia of radial glial cells in the dorsal and ventral regions of the embryonic brain. From this dataset, we identified cohorts of new molecules intrinsic to the cilia at distinct regions of the developing brain. We validated the cilium localization of several components of the translation machinery and studied the mechanistic roles of ciliary candidates previously linked to brain malformation, including Marcks, a key regulator of radial glial polarity, and Ckap2l, a protein associated with Filippi syndrome. These results revealed previously unrecognized ciliary mechanisms that regulate brain development. Thus, our brain proteomic dataset provides a unique resource for understanding ciliary functions in brain development and the molecular etiology of developmental disorders. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-28 |
| AnnouncementXML | Submission_2026-03-28_14:44:48.467.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sabyasachi Baboo |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | TMT6plex; S-carboxamidomethyl-L-cysteine |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-04-09 15:09:32 | ID requested | |
| ⏵ 1 | 2026-03-28 14:44:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cilia, cortex, TurboID, radial glia, embryonic brain, protein translation, proximity labeling, TMT quantitation, DatasetType:Proteomics |
Contact List
| John R. Yates III | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | jyates@scripps.edu |
| lab head | |
| Sabyasachi Baboo | |
| contact affiliation | The Scripps Research Institute, La Jolla, CA |
| contact email | sbaboo@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097579/ |
