PXD062786-1

PXD062786 is an original dataset announced via ProteomeXchange.

Title	SPO1 PISA assay to discover 33cGAMP binding proteins
Description	Cyclic oligonucleotide-based antiviral signaling systems (CBASS) are bacterial anti-phage defense operons that use nucleotide signals to control immune activation. Here we biochemically screen 57 diverse E. coli and Bacillus phages for the ability to disrupt CBASS immunity and discover anti-CBASS 4 (Acb4) from the Bacillus phage SPO1 as the founding member of a large family of >1,300 immune evasion proteins. A 2.1 Å crystal structure of Acb4 in complex with 3′3′-cGAMP reveals a tetrameric assembly that functions as a sponge to sequester CBASS signals and inhibit immune activation. We demonstrate Acb4 alone is sufficient to disrupt CBASS activation in vitro and enable immune evasion in vivo. Analyzing phages that infect diverse bacteria, we explain how Acb4 selectively targets nucleotide signals in host defense and avoids disruption of cellular homeostasis. Together, our results reveal principles of immune evasion protein evolution and explain a major mechanism phages use to inhibit host immunity.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-25_12:35:07.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nils Burger
SpeciesList	scientific name: Bacillus phage SPO1; NCBI TaxID: 2884427;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-09 11:07:13	ID requested
⏵ 1	2025-08-25 12:35:07	announced

10.1016/J.MOLCEL.2025.07.016;

submitter keyword: LC-MS, SPO1, PISA

Dr. Edward
contact affiliation	Dana-Farber Cancer Institute, Harvard Medical School, Howard Hughes Medical Institute
contact email	EdwardT_Chouchani@dfci.harvard.edu
lab head
Nils Burger
contact affiliation	Dana-Farber Cancer Institute and Harvard Medical School
contact email	nils_burger@dfci.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062786
     1. Label: PRIDE project
     2. Name: SPO1 PISA assay to discover 33cGAMP binding proteins