PXD062774-1

PXD062774 is an original dataset announced via ProteomeXchange.

Title	Context-dependent phosphorylation of CSDE1 drives interactions with ribosomes
Description	The RNA-binding protein CSDE1 is a key regulator of mRNA stability and translation in a broad spectrum of biological processes. We have previously shown that CSDE1 functions as an oncoprotein promoting invasion and metastasis in melanoma, whereas it behaves as a tumour suppressor promoting cellular senescence in squamous cell carcinoma. The reasons underlying these context-specific behaviours are unknown. To identify melanoma-specific vulnerabilities, we have compared CSDE1 protein isoforms and post-translational modifications in melanoma cells and keratinocytes, and in melanocytic cells of different tumorigenic potential. By combining long-read Nanopore sequencing with two-dimensional gel electrophoresis and transcriptome analysis, we identify one major isoform expressed in melanoma cells and patient samples. This isoform is phosphorylated early during cellular transformation, correlating with changes in its subcellular localization. We provide extensive interactome analysis of mammalian CSDE1, showing increased interactions with ribosomes in melanoma cells compared to healthy melanocytes. Importantly, interactions of CSDE1 with the ribosome are promoted by CSDE1 phosphorylation. Our data uncover a specific feature of melanoma cells that could be harnessed for therapeutic intervention
HostingRepository	PRIDE
AnnounceDate	2025-09-10
AnnouncementXML	Submission_2025-09-10_05:05:05.949.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Chiva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-09 00:51:06	ID requested
⏵ 1	2025-09-10 05:05:11	announced

Ciocia A, Guitart T, Mestre-Farr, à, s N, Nogales A, Milenkovic I, Coll O, Medina R, Chiva C, Sabid, ò E, Novoa EM, Gebauer F, Context-dependent phosphorylation of CSDE1 drives interactions with ribosomes. RNA, 31(11):1652-1666(2025) [pubmed]

10.1261/rna.080604.125;

submitter keyword: CSDE1,melanoma, phosphorylation

Fátima Gebauer
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr Aiguader 88, 08003 Barcelona, Spain; Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.
contact email	fatima.gebauer@crg.eu
lab head
Cristina Chiva
contact affiliation	University Pompeu Fabra
contact email	cristina.chiva@upf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062774

PRIDE project URI

• PRIDE
  1. PXD062774
     1. Label: PRIDE project
     2. Name: Context-dependent phosphorylation of CSDE1 drives interactions with ribosomes