PXD062750-1

PXD062750 is an original dataset announced via ProteomeXchange.

Title	Cobalt-induced stress reveals a prominent role of CzcC on the proteomic profile of Gluconacetobacter diazotrophicus PAL5
Description	Heavy metal accumulation in agricultural areas is a global environmental problem that affects microorganisms and plants, with serious implications for human health. This study aimed to investigate the molecular responses of the plant growth-promoting bacterium Gluconacetobacter diazotrophicus PAL5 to cobalt stress. We evaluated bacterial growth and cell viability under cobalt stress and performed comparative proteomic and reverse genetics analyses. Cobalt significantly inhibited bacterial growth but did not cause cell death. Proteomic analysis in the presence of 2.5 mM CoCl2, which caused approximately 50% growth inhibition, revealed the induction of pathways related to iron uptake, carbohydrate metabolism, amino acid metabolism, quality control, and efflux. Knockout mutants for genes involved in these pathways (∆tbdR, ∆zwf, ∆pdhB, ∆argH and ∆czcC) confirmed the essential role of the CzcC efflux system in cobalt tolerance. Cobalt stress triggers molecular responses in G. diazotrophicus PAL5, with efflux systems playing a crucial role in stress tolerance.
HostingRepository	PRIDE
AnnounceDate	2025-06-03
AnnouncementXML	Submission_2025-06-02_20:08:37.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gonçalo Souza Filho
SpeciesList	scientific name: Gluconacetobacter diazotrophicus PA1 5; NCBI TaxID: 272568;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 11:41:53	ID requested
⏵ 1	2025-06-02 20:08:38	announced

10.1093/JAMBIO/LXAF113;

submitter keyword: endophytic bacteria, extracytoplasmic proteins,Efflux system, cobalt stress, LC-MS/MS

Gonçalo Apolinário
contact affiliation	Laboratório de Biotecnologia (Unidade de Biologia Integrativa, Setor de Genômica e Proteômica), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, Rio de Janeiro, Brazil
contact email	goncalos@uenf.br
lab head
Gonçalo Souza Filho
contact affiliation	State University of Northern Rio de Janeiro (UENF)
contact email	goncalos@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD062750

PRIDE project URI

• PRIDE
  1. PXD062750
     1. Label: PRIDE project
     2. Name: Cobalt-induced stress reveals a prominent role of CzcC on the proteomic profile of Gluconacetobacter diazotrophicus PAL5