PXD062748-1

PXD062748 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis reveal early event molecular effectors of anti-CD19 CAR-T cell therapy in hematological cancer
Description	Chimeric antigen receptor T-cell (CAR-T) therapy is at the forefront of cell immunotherapy. In this study, we generated an anti-CD19 CAR-Jurkat T cell line using a locally produced second-generation CD19 CAR construct, enabling us to analyze early proteomic changes crucial for understanding the signaling pathways and mechanisms of action of this CAR-T cell. SILAC-heavy tagged RAJI B-cells and anti-CD19 CAR-Jurkat T-cells were co-cultured for ten minutes. The proteomic profiles were obtained via DIA methodology on the Orbitrap Astral LC-MS/MS platform. The proteome was extensively covered, resulting in approximately 8800 protein identifications at 1% FDR. The effector CAR-Jurkat cells showed proteomic changes involving antigen presentation by CD74. The target RAJI B-cells exhibited more significant alterations, such as CD28, essential for T-cell survival and activation.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_12:40:17.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Oluwafemi Teibo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled L-lysine; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 11:08:56	ID requested
⏵ 1	2025-07-30 12:40:18	announced

10.1016/J.JPROT.2025.105507;

submitter keyword: Effectors,SILAC, Proteomics, and Hematological Cancer.,CAR-T cell

Vitor Marcel
contact affiliation	Department of Biochemistry and Immunology, Ribeirão PretoMedical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
contact email	vitor.faca@fmrp.usp.br
lab head
John Oluwafemi Teibo
contact affiliation	University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
contact email	johnteibo@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD062748

PRIDE project URI

• PRIDE
  1. PXD062748
     1. Label: PRIDE project
     2. Name: Proteomics analysis reveal early event molecular effectors of anti-CD19 CAR-T cell therapy in hematological cancer