PXD062727-1

PXD062727 is an original dataset announced via ProteomeXchange.

Title	hDPSC secretome restores cognitive function and synaptic plasticity by reducing ROS and alleviating neuroinflammation through M2 microglial polarization after ischemic stroke
Description	Ischemic stroke triggers a cascade of oxidative stress, neuroinflammation, and synaptic dysfunction, leading to long-term cognitive and motor deficits. The human dental pulp stem cell (hDPSC) secretome, derived from neural crest-origin cells, has emerged as a promising cell-free therapeutic candidate due to its composition of neurotrophic, antioxidant, and immunomodulatory factors. In this study, we demonstrate that hDPSC secretome exerts neuroprotective effects in both a photothrombotic mouse model of ischemic stroke and CoCl₂-induced hypoxic BV2 microglial cells. Proteomic analysis revealed that the hDPSC secretome contains key antioxidant enzymes (SOD2, GSR, and GSTP1) and M2 microglial inducers (GRN, CSF1, and LRP1), which reduce neuronal ROS levels and promote anti-inflammatory microglial polarization. Furthermore, the hDPSC secretome enhanced neurogenesis and angiogenesis in the hippocampus and cortex, and restored synaptic structure and function by upregulating calcium signaling and ROBO–Rho pathway-associated synaptic proteins. These molecular and cellular effects translated into significant recovery of stroke-induced cognitive deficits, including improved memory and spatial learning, reduced anxiety-like behavior, as indicated by improved stress response, and enhanced motor function, reflected in improved coordination and balance. Together, these findings support the therapeutic potential of hDPSC secretome to promote brain repair after ischemic stroke by re-establishing redox balance, suppressing inflammation, and enhancing synaptic plasticity.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:53:53.060.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sehoon Park
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 05:36:09	ID requested
⏵ 1	2026-09-06 16:53:54	announced

10.1002/advs.76717;

Seong KJ, Park S, Bae SW, Kim D, Lee JH, Choi WS, Park ZY, Jung JY, Kim WJ, Human Dental Pulp Stem Cell Secretome Restores Ischemic Stroke-Impaired Motor and Cognitive Functions by Reprogramming Redox and Inflammatory Signaling. Adv Sci (Weinh), ():e76717(2026) [pubmed]

submitter keyword: None

Zee-Yong Park
contact affiliation	School of Life Sciences, Gwangju Institute of Science and Technology, 123 Cheomdangwagiro, Buk-gu, Gwangju 61005, Republic of Korea.
contact email	zeeyong@gist.ac.kr
lab head
Sehoon Park
contact affiliation	GIST
contact email	sehoon94@gist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD062727

PRIDE project URI

• PRIDE
  1. PXD062727
     1. Label: PRIDE project
     2. Name: hDPSC secretome restores cognitive function and synaptic plasticity by reducing ROS and alleviating neuroinflammation through M2 microglial polarization after ischemic stroke