PXD062673-1

PXD062673 is an original dataset announced via ProteomeXchange.

Title	Proteomic Changes in Cancer Cell Lines as a Result of Bacterial Infection
Description	This study investigates proteomic changes in two human cancer cell lines—A375 (melanoma) and OVCAR3 (ovarian cancer)—after infection with either Staphylococcus aureus (USA300 wildtype or ΔfnbAB mutant) or Salmonella enterica (SL1344). Using label-free quantitative proteomics, we observed that bacterial infections induced widespread remodeling of the host proteome, with mitochondrial and stress-related proteins generally upregulated, and proteins involved in DNA repair, cytoskeletal structure, and vesicle trafficking downregulated. The study highlights how bacterial invasion influences cancer cell responses.
HostingRepository	PRIDE
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-20_23:07:03.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Ren
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; NCBI TaxID: NEWT:216597; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Staphylococcus aureus (strain USA300); NCBI TaxID: NEWT:367830;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-07 01:25:19	ID requested
⏵ 1	2025-10-20 23:07:04	announced

10.1002/PMIC.70062;

submitter keyword: proteomics, OVCAR3, Staphylococcus aureus, Salmonella enterica, bacteria infection,A375, LC-MS/MS

David Goodlett
contact affiliation	1 Biochemistry & Microbiology, University of Victoria, Victoria, BC, Canada 2 Genome BC Proteomics Centre, University of Victoria, Victoria, BC, Canada
contact email	goodlett@uvic.ca
lab head
Bo Ren
contact affiliation	University of Victoria
contact email	boren@uvic.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062673

PRIDE project URI

• PRIDE
  1. PXD062673
     1. Label: PRIDE project
     2. Name: Proteomic Changes in Cancer Cell Lines as a Result of Bacterial Infection