PXD062623-1

PXD062623 is an original dataset announced via ProteomeXchange.

Title	Changes in histone abundance and post-translational modifications in Rana [Lithobates] catesbeiana tissues during temperature-mediated thyroid hormone-induced metamorphosis
Description	Amphibian metamorphosis involves thyroid hormone (TH)-dependent postembryonic remodeling of differentiated tissues as a tadpole transforms into a frog. Rana [Lithobates] catesbeiana (American bullfrog) metamorphosis can be precociously induced in premetamorphic tadpoles by treatment with exogenous TH. However, metamorphosis is temperature-dependent and does not occur at 5°C, even with TH treatment. Remarkably, exposure to TH at 5°C establishes a molecular memory that primes the tadpole for an accelerated metamorphosis when shifted to a permissive temperature (24°C). Previous research suggests that histone post-translational modifications (PTMs) and the incorporation of histone variants are altered upon cold temperature exposure in the presence and absence of TH. Herein, we use mass spectrometry to analyze the histone composition of premetamorphic R. catesbeiana blood, liver, and tailfin following TH injection at a permissive temperature (24°C), a non-permissive temperature (5°C), and a shifted condition (5 to 24°C). We identify changes in histone and non-histone chromatin associated protein abundance and putative PTMs, suggesting that epigenetic variations may contribute to early TH signaling events and to molecular memory.
HostingRepository	PRIDE
AnnounceDate	2025-04-25
AnnouncementXML	Submission_2025-04-25_10:38:58.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haley Kuecks-Winger
SpeciesList	scientific name: Lithobates catesbeianus; NCBI TaxID: 8400;
ModificationList	monomethylated residue; trimethylated residue; phosphorylated residue; acetylated residue; dimethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 11:13:36	ID requested
⏵ 1	2025-04-25 10:38:58	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, histone, post-translational modification, TMT,American bullfrog, metamorphosis

Caren C.
contact affiliation	Department of Biochemistry and Microbiology University of Victoria, Canada
contact email	chelbing@uvic.ca
lab head
Haley Kuecks-Winger
contact affiliation	University of Victoria
contact email	hkuecksw@uvic.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062623
     1. Label: PRIDE project
     2. Name: Changes in histone abundance and post-translational modifications in Rana [Lithobates] catesbeiana tissues during temperature-mediated thyroid hormone-induced metamorphosis